Antibacterial and Wound Healing Potential of Ethanolic Extract of <i>Zingiber Officinale</i> in Albino Rats

Aisha Hassan Balla Mohamed; Amira Altom Fawzi Osman

doi:doi:10.11648/j.jdmp.20170301.11

| Peer-Reviewed

Antibacterial and Wound Healing Potential of Ethanolic Extract of Zingiber Officinale in Albino Rats

Aisha Hassan Balla Mohamed, Amira Altom Fawzi Osman

Published in Journal of Diseases and Medicinal Plants (Volume 3, Issue 1)

Received: 25 November 2016 Accepted: 3 January 2017 Published: 24 January 2017

Views: Downloads:

Download PDF

Share This Article

Twitter
Linked In
Facebook

Abstract

Medicinal plants are used in a wide range in Sudan to treat several diseases, Zingiber officinale Roscoe is one of the plants that are central to the lives of traditional societies in Africa it has been reported as traditional folkloric medicine, the plant roots are used in some parts in Sudan for wound healing, this study aimed to assess the antibacterial and wound healing activity of Zingiber officinale ethanolic extract in wound infected albino rats and compare the effect of the plant against commercial reference drug against Staphylococcus aureus, methods of this study included fifteen swiss wistar albino rat, divided into three groups of five rats (Group 1 (wounded +infection, Group 2 wound +infection+ fusiderm ointment, Group 3 wound + infection +12% ethanol extract of Zingiber officinale with soft yellow paraffin), all results included microbial examination for bacterial count, histological examination for the presence of inflammatory cells and collagen and healing percentage measured by transparent ruler, the results of in vitro antimicrobial activity of the extract gave clear zones of inhibition on the standard strains of Proteus mirabilis, Klebsiellia pneumoniae, Escherichia coli, Pseudomonas aeruginosa and Staphylococcus aureus, significant reduction of bacterial number observed in ginger treated group from.5×10⁸cfu/ml to.5×10² cfu/ml on day 8 of dressing while the positive control group showed bacterial count reduction from.5×10⁸cfu/ml to complete diminishing of bacterial infection. Significant difference in the wound closure was observed in positive control group (fusiderm) to give a percentage of 55.7% on day 4 and 91% on day 8. The ginger treatment group also showed faster wound healing with a percentage closed to positive control 51.4% on day 4 and 90.6% on day 8, while the wound healing rate was slow and took more than 8 days in negative control, histological examination of granulated tissue done by Haematoxylin and Eosin stain and van Gieson stain showed more collagen fibers formation and reduction of inflammatory cell during the healing period, finally the study concluded that Zingiber officinale ethanolic extract exert antibacterial and wound healing capacity, this study established a good support to the use of plants in herbal medicine and as abase for the development of new drugs.

Published in	Journal of Diseases and Medicinal Plants (Volume 3, Issue 1)
DOI	10.11648/j.jdmp.20170301.11
Page(s)	1-6
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2017. Published by Science Publishing Group

Keywords

Wound Infection, Zingiber Officinale, Ginger, Anti-bacterial Activity, Healing Activity

References

[1]	Kumar, B.; Vijayakumar, M.; Govindarajan, R.; Pushpangadan, P. Ethno pharmacological approaches to wound Healing Exploring medicinal plants of India. J. Ethnopharmacol. 2007, 114, 103–113.
[2]	Bowler PG. The anaerobic and aerobic microbiology of wounds: A review. Wounds 1998; 10: 170.
[3]	Bowler PG, Davies BJ. The microbiology of acute and chronic wounds. Wounds 1999; 11: 72.
[4]	Robson MC. Wound infection. A failure of wound healing caused by an imbalance of bacteria. Surg Clin N Am 1997;77: 637.
[5]	Griffith-Jones A. Methicillin resistant Staphylococcus aureus in wound care. J Wound Care 1995; 4: 481.
[6]	Akroum S, Satta D and Lalauoui K. (2009) Antibacterial antioxidant, cytotoxic activities and phytochemical screening of some Algerian plants. Euro. J Sci. Res., 31 (2), pp. 289-295.
[7]	Mona A Mohamed. Antimicrobial agent, preparation of extract in biological activity of Nigella Sativa. Egypt, Cairo2008; page20.
[8]	Singh G. Studies on the Biocidal activities of certain essential oils. Journal of Medicinal and Aromatic plant Sciences 1999; 21 (4) 1119-1123.
[9]	Tan B. K. H and Vanitha J; Immunomodulatory and Antimicrobial Effects of Some Traditional Chinese Medicinal Herbs; A Review, Current Medicinal Chemistry, Vol. 11, No. 11, 2004, pp. 1423-1430.
[10]	Zaika L L; “Spices and Herbs. Their antimicrobial activity and its determination;” Journal of Food Safety, Vol. 9, No. 21, 1975, pp. 97-118.
[11]	Opdyke DL J; Food Cosmet. Toxicology 1974; 12 (Suppl.) PP. 901.
[12]	Gao D, Zhang Y. Comparative antibacterial activities of crude polysaccharides and flavonoids from Zingiber officinale and their extraction. Asian j Trad Med.2010; 5: 235–238.
[13]	Cooper K E and Kavanagh. F. (ED). Analytical microbiology. Academic Press, New York Vol. 1. 1972.
[14]	Tilton R C and Howard B J. Antimicrobial susceptibility testing In Carson D, Birchor S, Clinical and pathogenic Microbiology. Mosby Co, St Louis, pp 121_115.
[15]	Carter, S. L. Cooper and Gunn's Dispensing for Pharmaceutical Students; CBS Publisher and Distributors: Delhi, India, 1997; pp. 199–200.
[16]	Abd Allah, A. N. (2004). Antimicrobial and wound healing activity of ten selected medicinal plants. unbuplished.
[17]	European journal of applied microbiology and biotechnology Volume 9, Issue1, pp 59-62.
[18]	Mcmanus, J. G. A.; Mowry, R. W. Staining Methods: Histological and Histochemical; Harper and Row: New York, NY, USA, 1984.
[19]	Ekwenye U N, department of microbiology; Michael Okapra University of agriculture, Umudike. P. M. B 7267 Umuahia, Abia state. Nigera, international journal of molecular medicine and advance science 2005; 1 (4): 416.
[20]	Roscoe L. Warner, a combination of curcumin and ginger extract improves abrasion wound healing of corticosteroid damaged hairless rat skin, Wound Repair Regen. 2009; 17 (3): 360.
[21]	Sreenivasan Sasidharan, Wound Healing Potential of Elaeis guineensis Jacq Leaves in an Infected Albino Rat Mode, Molecules 2010, 15, 3186-3199.
[22]	Muthusamy S Kumar 2008, Triphala Promotes Healing of Infected Full-Thickness Dermal Wound, Journal of Surgical Research 144, 94–101.

Cite This Article

Plain Text BibTeX RIS

APA Style

Aisha Hassan Balla Mohamed, Amira Altom Fawzi Osman. (2017). Antibacterial and Wound Healing Potential of Ethanolic Extract of Zingiber Officinale in Albino Rats. Journal of Diseases and Medicinal Plants, 3(1), 1-6. https://doi.org/10.11648/j.jdmp.20170301.11

Copy | Download

ACS Style

Aisha Hassan Balla Mohamed; Amira Altom Fawzi Osman. Antibacterial and Wound Healing Potential of Ethanolic Extract of Zingiber Officinale in Albino Rats. J. Dis. Med. Plants 2017, 3(1), 1-6. doi: 10.11648/j.jdmp.20170301.11

Copy | Download

AMA Style

Aisha Hassan Balla Mohamed, Amira Altom Fawzi Osman. Antibacterial and Wound Healing Potential of Ethanolic Extract of Zingiber Officinale in Albino Rats. J Dis Med Plants. 2017;3(1):1-6. doi: 10.11648/j.jdmp.20170301.11

Copy | Download

@article{10.11648/j.jdmp.20170301.11,
  author = {Aisha Hassan Balla Mohamed and Amira Altom Fawzi Osman},
  title = {Antibacterial and Wound Healing Potential of Ethanolic Extract of Zingiber Officinale in Albino Rats},
  journal = {Journal of Diseases and Medicinal Plants},
  volume = {3},
  number = {1},
  pages = {1-6},
  doi = {10.11648/j.jdmp.20170301.11},
  url = {https://doi.org/10.11648/j.jdmp.20170301.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.jdmp.20170301.11},
  abstract = {Medicinal plants are used in a wide range in Sudan to treat several diseases, Zingiber officinale Roscoe is one of the plants that are central to the lives of traditional societies in Africa it has been reported as traditional folkloric medicine, the plant roots are used in some parts in Sudan for wound healing, this study aimed to assess the antibacterial and wound healing activity of Zingiber officinale ethanolic extract in wound infected albino rats and compare the effect of the plant against commercial reference drug against Staphylococcus aureus, methods of this study included fifteen swiss wistar albino rat, divided into three groups of five rats (Group 1 (wounded +infection, Group 2 wound +infection+ fusiderm ointment, Group 3 wound + infection +12% ethanol extract of Zingiber officinale with soft yellow paraffin), all results included microbial examination for bacterial count, histological examination for the presence of inflammatory cells and collagen and healing percentage measured by transparent ruler, the results of in vitro antimicrobial activity of the extract gave clear zones of inhibition on the standard strains of Proteus mirabilis, Klebsiellia pneumoniae, Escherichia coli, Pseudomonas aeruginosa and Staphylococcus aureus, significant reduction of bacterial number observed in ginger treated group from.5×108cfu/ml to.5×102 cfu/ml on day 8 of dressing while the positive control group showed bacterial count reduction from.5×108cfu/ml to complete diminishing of bacterial infection. Significant difference in the wound closure was observed in positive control group (fusiderm) to give a percentage of 55.7% on day 4 and 91% on day 8. The ginger treatment group also showed faster wound healing with a percentage closed to positive control 51.4% on day 4 and 90.6% on day 8, while the wound healing rate was slow and took more than 8 days in negative control, histological examination of granulated tissue done by Haematoxylin and Eosin stain and van Gieson stain showed more collagen fibers formation and reduction of inflammatory cell during the healing period, finally the study concluded that Zingiber officinale ethanolic extract exert antibacterial and wound healing capacity, this study established a good support to the use of plants in herbal medicine and as abase for the development of new drugs.},
 year = {2017}
}

Copy | Download

TY  - JOUR
T1  - Antibacterial and Wound Healing Potential of Ethanolic Extract of Zingiber Officinale in Albino Rats
AU  - Aisha Hassan Balla Mohamed
AU  - Amira Altom Fawzi Osman
Y1  - 2017/01/24
PY  - 2017
N1  - https://doi.org/10.11648/j.jdmp.20170301.11
DO  - 10.11648/j.jdmp.20170301.11
T2  - Journal of Diseases and Medicinal Plants
JF  - Journal of Diseases and Medicinal Plants
JO  - Journal of Diseases and Medicinal Plants
SP  - 1
EP  - 6
PB  - Science Publishing Group
SN  - 2469-8210
UR  - https://doi.org/10.11648/j.jdmp.20170301.11
AB  - Medicinal plants are used in a wide range in Sudan to treat several diseases, Zingiber officinale Roscoe is one of the plants that are central to the lives of traditional societies in Africa it has been reported as traditional folkloric medicine, the plant roots are used in some parts in Sudan for wound healing, this study aimed to assess the antibacterial and wound healing activity of Zingiber officinale ethanolic extract in wound infected albino rats and compare the effect of the plant against commercial reference drug against Staphylococcus aureus, methods of this study included fifteen swiss wistar albino rat, divided into three groups of five rats (Group 1 (wounded +infection, Group 2 wound +infection+ fusiderm ointment, Group 3 wound + infection +12% ethanol extract of Zingiber officinale with soft yellow paraffin), all results included microbial examination for bacterial count, histological examination for the presence of inflammatory cells and collagen and healing percentage measured by transparent ruler, the results of in vitro antimicrobial activity of the extract gave clear zones of inhibition on the standard strains of Proteus mirabilis, Klebsiellia pneumoniae, Escherichia coli, Pseudomonas aeruginosa and Staphylococcus aureus, significant reduction of bacterial number observed in ginger treated group from.5×108cfu/ml to.5×102 cfu/ml on day 8 of dressing while the positive control group showed bacterial count reduction from.5×108cfu/ml to complete diminishing of bacterial infection. Significant difference in the wound closure was observed in positive control group (fusiderm) to give a percentage of 55.7% on day 4 and 91% on day 8. The ginger treatment group also showed faster wound healing with a percentage closed to positive control 51.4% on day 4 and 90.6% on day 8, while the wound healing rate was slow and took more than 8 days in negative control, histological examination of granulated tissue done by Haematoxylin and Eosin stain and van Gieson stain showed more collagen fibers formation and reduction of inflammatory cell during the healing period, finally the study concluded that Zingiber officinale ethanolic extract exert antibacterial and wound healing capacity, this study established a good support to the use of plants in herbal medicine and as abase for the development of new drugs.
VL  - 3
IS  - 1
ER  -

Copy | Download

Author Information

Aisha Hassan Balla Mohamed

Microbiology Department, Alzaiem Alazhari University (AAU), Khartoum, Sudan
Amira Altom Fawzi Osman

Microbiology Department, Faculty of Medical Laboratories, Alzaiem Alazhari University (AAU), Khartoum, Sudan

Download PDF

Sections

Plain Text BibTeX RIS

APA Style

Aisha Hassan Balla Mohamed, Amira Altom Fawzi Osman. (2017). Antibacterial and Wound Healing Potential of Ethanolic Extract of Zingiber Officinale in Albino Rats. Journal of Diseases and Medicinal Plants, 3(1), 1-6. https://doi.org/10.11648/j.jdmp.20170301.11

Copy | Download

ACS Style

Aisha Hassan Balla Mohamed; Amira Altom Fawzi Osman. Antibacterial and Wound Healing Potential of Ethanolic Extract of Zingiber Officinale in Albino Rats. J. Dis. Med. Plants 2017, 3(1), 1-6. doi: 10.11648/j.jdmp.20170301.11

Copy | Download

AMA Style

Aisha Hassan Balla Mohamed, Amira Altom Fawzi Osman. Antibacterial and Wound Healing Potential of Ethanolic Extract of Zingiber Officinale in Albino Rats. J Dis Med Plants. 2017;3(1):1-6. doi: 10.11648/j.jdmp.20170301.11

Copy | Download

@article{10.11648/j.jdmp.20170301.11,
  author = {Aisha Hassan Balla Mohamed and Amira Altom Fawzi Osman},
  title = {Antibacterial and Wound Healing Potential of Ethanolic Extract of Zingiber Officinale in Albino Rats},
  journal = {Journal of Diseases and Medicinal Plants},
  volume = {3},
  number = {1},
  pages = {1-6},
  doi = {10.11648/j.jdmp.20170301.11},
  url = {https://doi.org/10.11648/j.jdmp.20170301.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.jdmp.20170301.11},
  abstract = {Medicinal plants are used in a wide range in Sudan to treat several diseases, Zingiber officinale Roscoe is one of the plants that are central to the lives of traditional societies in Africa it has been reported as traditional folkloric medicine, the plant roots are used in some parts in Sudan for wound healing, this study aimed to assess the antibacterial and wound healing activity of Zingiber officinale ethanolic extract in wound infected albino rats and compare the effect of the plant against commercial reference drug against Staphylococcus aureus, methods of this study included fifteen swiss wistar albino rat, divided into three groups of five rats (Group 1 (wounded +infection, Group 2 wound +infection+ fusiderm ointment, Group 3 wound + infection +12% ethanol extract of Zingiber officinale with soft yellow paraffin), all results included microbial examination for bacterial count, histological examination for the presence of inflammatory cells and collagen and healing percentage measured by transparent ruler, the results of in vitro antimicrobial activity of the extract gave clear zones of inhibition on the standard strains of Proteus mirabilis, Klebsiellia pneumoniae, Escherichia coli, Pseudomonas aeruginosa and Staphylococcus aureus, significant reduction of bacterial number observed in ginger treated group from.5×108cfu/ml to.5×102 cfu/ml on day 8 of dressing while the positive control group showed bacterial count reduction from.5×108cfu/ml to complete diminishing of bacterial infection. Significant difference in the wound closure was observed in positive control group (fusiderm) to give a percentage of 55.7% on day 4 and 91% on day 8. The ginger treatment group also showed faster wound healing with a percentage closed to positive control 51.4% on day 4 and 90.6% on day 8, while the wound healing rate was slow and took more than 8 days in negative control, histological examination of granulated tissue done by Haematoxylin and Eosin stain and van Gieson stain showed more collagen fibers formation and reduction of inflammatory cell during the healing period, finally the study concluded that Zingiber officinale ethanolic extract exert antibacterial and wound healing capacity, this study established a good support to the use of plants in herbal medicine and as abase for the development of new drugs.},
 year = {2017}
}

Copy | Download

TY  - JOUR
T1  - Antibacterial and Wound Healing Potential of Ethanolic Extract of Zingiber Officinale in Albino Rats
AU  - Aisha Hassan Balla Mohamed
AU  - Amira Altom Fawzi Osman
Y1  - 2017/01/24
PY  - 2017
N1  - https://doi.org/10.11648/j.jdmp.20170301.11
DO  - 10.11648/j.jdmp.20170301.11
T2  - Journal of Diseases and Medicinal Plants
JF  - Journal of Diseases and Medicinal Plants
JO  - Journal of Diseases and Medicinal Plants
SP  - 1
EP  - 6
PB  - Science Publishing Group
SN  - 2469-8210
UR  - https://doi.org/10.11648/j.jdmp.20170301.11
AB  - Medicinal plants are used in a wide range in Sudan to treat several diseases, Zingiber officinale Roscoe is one of the plants that are central to the lives of traditional societies in Africa it has been reported as traditional folkloric medicine, the plant roots are used in some parts in Sudan for wound healing, this study aimed to assess the antibacterial and wound healing activity of Zingiber officinale ethanolic extract in wound infected albino rats and compare the effect of the plant against commercial reference drug against Staphylococcus aureus, methods of this study included fifteen swiss wistar albino rat, divided into three groups of five rats (Group 1 (wounded +infection, Group 2 wound +infection+ fusiderm ointment, Group 3 wound + infection +12% ethanol extract of Zingiber officinale with soft yellow paraffin), all results included microbial examination for bacterial count, histological examination for the presence of inflammatory cells and collagen and healing percentage measured by transparent ruler, the results of in vitro antimicrobial activity of the extract gave clear zones of inhibition on the standard strains of Proteus mirabilis, Klebsiellia pneumoniae, Escherichia coli, Pseudomonas aeruginosa and Staphylococcus aureus, significant reduction of bacterial number observed in ginger treated group from.5×108cfu/ml to.5×102 cfu/ml on day 8 of dressing while the positive control group showed bacterial count reduction from.5×108cfu/ml to complete diminishing of bacterial infection. Significant difference in the wound closure was observed in positive control group (fusiderm) to give a percentage of 55.7% on day 4 and 91% on day 8. The ginger treatment group also showed faster wound healing with a percentage closed to positive control 51.4% on day 4 and 90.6% on day 8, while the wound healing rate was slow and took more than 8 days in negative control, histological examination of granulated tissue done by Haematoxylin and Eosin stain and van Gieson stain showed more collagen fibers formation and reduction of inflammatory cell during the healing period, finally the study concluded that Zingiber officinale ethanolic extract exert antibacterial and wound healing capacity, this study established a good support to the use of plants in herbal medicine and as abase for the development of new drugs.
VL  - 3
IS  - 1
ER  -

Copy | Download