Developing nations are experiencing energy deficit because of overdependence on fossil-based fuels. Countries such as Nigeria have abundant raw materials for biofuels, yet these have not been explored. This study was designed to evaluate the bioethanol production potentials of lignocellulosic-based wastes. The mean glucose yield and TRS obtained from the 13.1M H2SO4 were significantly higher than those of 9.4M and 5.6M H2SO4 hydrolysis. The mean glucose yield and TRS obtained from the 13.1M H2SO4 hydrolysis were: CP (85.1±5.7, 209.8±3.7mg/kg), YP (269.2±11.2, 541.3±7.8 mg/kg), PP (304.0±6.1, 461.2±3.6 mg/kg) and SD (343.2±4.8, 535.9±5.0 mg/kg). The 13.1M hydrolysate was used for the ethanol production and the maximum production was obtained at 48hours of fermentation, the mean ethanol yield being: CP - 160.0±15.1 mL/kg, YP -211.7±15.3 mL/kg, PP - 265.0±20.5 mL/kg and SD - 280.0±11.5 mL/kg. A linear relationship exists between the ethanol yield and fermentation time (R2 = 0.711). Sawdust produced the highest glucose and ethanol yield among the substrates; hence ethanol production from sawdust should be explored and optimized.
Published in | International Journal of Sustainable and Green Energy (Volume 4, Issue 4) |
DOI | 10.11648/j.ijrse.20150404.13 |
Page(s) | 141-149 |
Creative Commons |
This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited. |
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Copyright © The Author(s), 2015. Published by Science Publishing Group |
Bioethanol Production, Glucose Yield, Lignocellulosic Wastes, Saccharomyces Cerevisiae, Total Reducing Sugars (TRS)
[1] | Tripetchkul S, Hillary ZD, and Ishizaki A. (1998) strategies for improving ethanol Production using Z. Mobilis. Recent Research Developments in Agricultural and Biological Chemistry 2:41-55. |
[2] | Nomura M, Bin T, and Nakao S. I, (2002). Selective ethanol extraction from fermentation broth using a silicate membrane. Separation purifying Technology. 27:59-66. |
[3] | Green, Harvey (2006) Wood: Craft, Culture, History Penguin Books, New York, Page 403. ISB 978-1-101-2-0185-5. |
[4] | Bassey, N. (2010). Oil Politics: Nigeria’s Unacceptable Biofuels Policy. Retrieved from http://234next.com/csp/cms/sites/Next/Money/5643461-183/story.csp, on August 15, 2011. |
[5] | Oniemola, P.K. & Sanusi, G. (2009). The Nigerian Bio-Fuel Policy and Incentives (2007): A Need to Follow the Brazilian Pathway. International Association for Energy Economics. |
[6] | Lynd, Lee R. Cushman, Janet, H., Nicholas, Roberta J., Wyman, Charles E. (2003) Fuel Ethanol from cellulosic Biomass: Science, New Series, Vol 251 No. 4999, pp1318-1323. |
[7] | Shilo H, and Neimo, L. (1975). The Structure and properties of Cellulose. In Proc. Symp on Enzymatic Hydrolysis of Cellulose, Aulanka, Finland, (ed. M. Baily, T. M Enaria and M, Linko), pp 9-21 SITRA. |
[8] | Yu B, Zhang F, Zheng Y, Wang P. (1994). Alcohol fermentation from the mash dried sweet potato with its dregs using co-immobilized yeast. Process Biochemistry 31 (1): 1-6. |
[9] | Yu B, Zhang H. (2002). Pretreatment of cellulose pyrosylate for ethanol production by Saccharomyces cerevisiae, Pischia sp. YZ-1 and Zymomonas mobilis. Biomass and Bioenergy 24: 257 – 267. |
[10] | Amutha R and Gunasekaran P. (2001). Production of ethanol from liquefied cassava starch using co- immobilized cells of Zymomonas mobilis and Saccharomyces diastaticus. Journal of Biosciences and Bioengineering 92(6): 560-564. |
[11] | Fujita Y, Takahashi S, Ueda M, Tanaka A, Okada H, Morikawa Y, Kaqaguchi T, Arai M, Fukuda H, Kondo A. (2002). Direct and efficient production of ethanol from cellulosic material with a yeast strain displaying cellulolytic enzymes. Applied and environmental microbiology 68 (10): 5136 – 5141. |
[12] | Alfenore S, Jouve CM, Guillout SE, Uribelarrea JL, Goma G, and Benbalis L. (2002). Improving ethanol production and viability of Saccharomyces cerevisiae by a vitamin feeding strategy during feed-batch process. In Applied Microbiology Biotechnology 60: 67 – 72. |
[13] | Deng M D and Coleman J R, (1999). Ethanol synthesis by genetic engineering in Cyanobacteria. Applied and Environmental Microbiology. 65(2): 523 – 528. |
[14] | Jirku V. (1999). Whole cell immobilization as a means of enhancing ethanol tolerance. Journal of Industrial Microbiology and Biotechnology 22:147 – 151. |
[15] | Association of Official Analytical Chemists (A.O.A.C, 1984, 1990, 1998). Official Methods of Analysis”. Association of Official Analytical Chemists (1984) Official Methods of Analysis, 14.023. A.O.A.C. |
[16] | Agarwal A.K (2005) Biofuels: In wealth from waste; trends and technologies. (ed Lal and Reedy), 2nd Edition. The Energy and Resources Instituted (TERI) Press. ISBNSI-7993-067-X. |
[17] | Farone W. A and Cuzens J. E. (1996a). Method of Producing sugars using strong acid hydrolysis of cellulosic and hemicellulosic materials. (US Patent No. 5562777) USA: Arkenol, Inc. |
[18] | Agu, R. C., Amadife, A.E., Ude, C. M., Onya, A., Ogu, E. O., Okafor, M. and Zejiofor,E (1997) “Combined heat treatment and acid hydrolysis of cassava grate waste (CGW). Biomass for ethanol production” waste management. 17(1), 91-96. |
[19] | Banerjee, N., Bhatnagar, R. and Viswanathan, L. (1981). “Inhibition of glycolysis by furfural in Saccharomyces cerevisiae”. European Journal of Applied Microbiology and Biotechnology. 11:226- 228. |
[20] | Jeffries, T.W. and Y.Y.Lee, (1999). Feedstocks new supplies and Processing. Applied Biochem. Biotechnol. 34:77-79. |
[21] | Bousssaid, A., J. Robinson, Y. Cai, D.J Gregg and J.N. Saddler, (1999). Fermentability of the Hemicelluloses – derived sugars from steam – exploded softwood (Douglas fir). Biotechnol. Bioeng., 64: 284-289. |
[22] | Betts WB, Dart R.K, Ball A.S. Pedlar S. L. (1991). Biosynthesis and Structure of Lignocelluloses and Synthetic Materials, Springer-Verlag, Berlin, Germany, pp. 139-155. |
[23] | Sun Y, Cheng J. (2002). Hydrolysis of Lignocellulosic Material from Ethanol Production: A review Biores. Technol. 83:1-11. |
[24] | Ojumu, T.V. B.E. Attah – Daniel. E. Betiku and B.O. Solomon, (2003). Auto – hydrolysisof lignocellulosics under extremely low sulphuric acid and high temperature conditionsin batch reactor. Biotechnol. Bioprocess Eng., 8:291-293. |
[25] | Badmus, M.A.O., (2002). Auto hydrolysis Production of glucose from palm tree trunk. Nig. J.Ind. Syst. Stud., 1: 1-4. |
[26] | Teerapatr S, Lerdluk K and La-aied S (2006). Approach of cassava Waste Pre-treatments for Fuel Ethanol production in Thailand. Biotechnology Department, Thailand Institute of Scientific and Technological Research (TISTR), 35 19003, Techno polis, Klong 5,Klong Luang, Pathumthani 12120, Thailand. |
[27] | Roberto J. C, Mussato S, J., Rodriguez RCLB (2003) Dilute-acid hydrolysis for optimization of xylose recovery from rice straw in a semi-pilot reactor. Indust. Crops Prod 17:171-176. |
[28] | Parajo JC. Dominquez HD, Dominquez JM (1998). Biotechnological production of xylitol. Part 1: interest of xylitol and fundamentals of biosynthesis. Biores, Technol 65:191-201. |
[29] | Licht, F.O (2006). “World ethanol markets: The outlook to 2015.” Tunbridge Wells Agra Europe special report UK. |
[30] | Chahal DS (1992). Bioconversion of polysaccharides of lignocelluloses and simultaneous degradation of lignin. In Kennedy et al. (eds) Lignocellulosics: Science, Technology, Development and Use. Ellis Horwood Limited, England, pp, 83 – 93. |
[31] | Delgenes J.P, Laplace J.M, Moletta R, Navarro J.M, Moletta R, Navarro J.M. (1996). Comparative study of separated fermentations and co-fermentation process to produce ethanol from hard wood derieved hydrolysate. Biomass and Bioenergy 11 (4): 353 – 360. |
[32] | Kadam K. L, Forest L H., Jacobson W. A., (2000). Rice Straw as a lignocellulosic resource collection, processing, transportation and environmental aspects. Biomass, Bioenergy, 2000. 8:369-389. |
[33] | Brooks T. A. and Ingram L. O. (1995) Conversion of mixed waste office paper to ethanol by genetically engineered Kebsilla Oxytoka strain P2. Biotechnology Progress 11:619-625. |
[34] | Doran JB, Aldrich HC, and Ingram LO. (1994). Saccharification and fermentation of Sugarcane bagasse by Klebsiella oxytoca P2 containing chromosomally integrated genes encoding the Zymomonas mobilis ethanol pathway. Biotechnology and Bioengineering 44:240-247. |
[35] | Moniruzzaman M, Dien BS, Ferrer B, Hespell RB, Dale BE, Ingram LO, Bothast RJ. (1996). Ethanol production from AFEX pretreated corn fiber by recombinant bacteria. Biotechnology Letters 18:985 – 990. |
[36] | Doran J. B., (ripe), Sutton M, Foster B. (2000). Fermentations of pectin-rich biomass withrecombinant bacteria to produce fuel ethanol. Applied Biochemistry and Biotechnology. 84-86:141-152. |
[37] | Dien B. S, Cotta M. A., and Jefferies T. W. (2003). Bacteria Engineered for Fuel Ethanol Production: Current Status: Applied Microbiology and Biotechnology 63(3):258-266. |
[38] | Dien BS, Hopspell RB, Wyckoff HA, Bothast RJ. (1998). Fermentation of hexose and pentose sugars using a novel ethanologenic Escherichia coli strain. Enzyme and Microbial Technology 23:366-371. |
[39] | Toma M, Kalnenieks U, Berzins A, Vigants A, Rikmains M, Viesturs U.(2002). Theeffect of mixing on glucose fermentation by Z.mobilis continuous culture Process Biochemistry:1-4. |
[40] | Jirku V. (1999). Whole cell immobilization as a means of enhancing ethanol tolerance. Journal of Industrial Microbiology and Biotechnology 22: 147 – 151. |
[41] | Bothast RJ, Nichols NN, and Dien BS. (1999). Fermentations with new recombinant organisms. Biotechnology Progress 15:867-875. |
[42] | Wiselogel A, Tyson S, and Johnson D. (1996). Biomass feedstock resources and composition. Handbook on Bioethanol: production and utilization, edited by CE Wyman [Applied Energy Technology Series], pp.105 – 118. |
APA Style
Ana Godson R. E. E., Sokan Adeaga Adewale Allen. (2015). Bio-Ethanol Yield from Selected Lignocellulosic Wastes. International Journal of Sustainable and Green Energy, 4(4), 141-149. https://doi.org/10.11648/j.ijrse.20150404.13
ACS Style
Ana Godson R. E. E.; Sokan Adeaga Adewale Allen. Bio-Ethanol Yield from Selected Lignocellulosic Wastes. Int. J. Sustain. Green Energy 2015, 4(4), 141-149. doi: 10.11648/j.ijrse.20150404.13
@article{10.11648/j.ijrse.20150404.13, author = {Ana Godson R. E. E. and Sokan Adeaga Adewale Allen}, title = {Bio-Ethanol Yield from Selected Lignocellulosic Wastes}, journal = {International Journal of Sustainable and Green Energy}, volume = {4}, number = {4}, pages = {141-149}, doi = {10.11648/j.ijrse.20150404.13}, url = {https://doi.org/10.11648/j.ijrse.20150404.13}, eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijrse.20150404.13}, abstract = {Developing nations are experiencing energy deficit because of overdependence on fossil-based fuels. Countries such as Nigeria have abundant raw materials for biofuels, yet these have not been explored. This study was designed to evaluate the bioethanol production potentials of lignocellulosic-based wastes. The mean glucose yield and TRS obtained from the 13.1M H2SO4 were significantly higher than those of 9.4M and 5.6M H2SO4 hydrolysis. The mean glucose yield and TRS obtained from the 13.1M H2SO4 hydrolysis were: CP (85.1±5.7, 209.8±3.7mg/kg), YP (269.2±11.2, 541.3±7.8 mg/kg), PP (304.0±6.1, 461.2±3.6 mg/kg) and SD (343.2±4.8, 535.9±5.0 mg/kg). The 13.1M hydrolysate was used for the ethanol production and the maximum production was obtained at 48hours of fermentation, the mean ethanol yield being: CP - 160.0±15.1 mL/kg, YP -211.7±15.3 mL/kg, PP - 265.0±20.5 mL/kg and SD - 280.0±11.5 mL/kg. A linear relationship exists between the ethanol yield and fermentation time (R2 = 0.711). Sawdust produced the highest glucose and ethanol yield among the substrates; hence ethanol production from sawdust should be explored and optimized.}, year = {2015} }
TY - JOUR T1 - Bio-Ethanol Yield from Selected Lignocellulosic Wastes AU - Ana Godson R. E. E. AU - Sokan Adeaga Adewale Allen Y1 - 2015/07/01 PY - 2015 N1 - https://doi.org/10.11648/j.ijrse.20150404.13 DO - 10.11648/j.ijrse.20150404.13 T2 - International Journal of Sustainable and Green Energy JF - International Journal of Sustainable and Green Energy JO - International Journal of Sustainable and Green Energy SP - 141 EP - 149 PB - Science Publishing Group SN - 2575-1549 UR - https://doi.org/10.11648/j.ijrse.20150404.13 AB - Developing nations are experiencing energy deficit because of overdependence on fossil-based fuels. Countries such as Nigeria have abundant raw materials for biofuels, yet these have not been explored. This study was designed to evaluate the bioethanol production potentials of lignocellulosic-based wastes. The mean glucose yield and TRS obtained from the 13.1M H2SO4 were significantly higher than those of 9.4M and 5.6M H2SO4 hydrolysis. The mean glucose yield and TRS obtained from the 13.1M H2SO4 hydrolysis were: CP (85.1±5.7, 209.8±3.7mg/kg), YP (269.2±11.2, 541.3±7.8 mg/kg), PP (304.0±6.1, 461.2±3.6 mg/kg) and SD (343.2±4.8, 535.9±5.0 mg/kg). The 13.1M hydrolysate was used for the ethanol production and the maximum production was obtained at 48hours of fermentation, the mean ethanol yield being: CP - 160.0±15.1 mL/kg, YP -211.7±15.3 mL/kg, PP - 265.0±20.5 mL/kg and SD - 280.0±11.5 mL/kg. A linear relationship exists between the ethanol yield and fermentation time (R2 = 0.711). Sawdust produced the highest glucose and ethanol yield among the substrates; hence ethanol production from sawdust should be explored and optimized. VL - 4 IS - 4 ER -