Phytochemical Constituents and Antioxidant Properties of Aqueous Leaf Extract of <i>Myrianthus arboreus</i>

Awodi Patience Ilecholubo; Abbah Christopher Okpachi; Ejembi Daniel Ocholi

doi:doi:10.11648/j.ijbse.20170501.12

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Phytochemical Constituents and Antioxidant Properties of Aqueous Leaf Extract of Myrianthus arboreus

Awodi Patience Ilecholubo, Abbah Christopher Okpachi, Ejembi Daniel Ocholi

Published in International Journal of Biomedical Science and Engineering (Volume 5, Issue 1)

Received: 17 January 2017 Accepted: 29 January 2017 Published: 22 February 2017

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Abstract

This study was carried out to determine the phytochemical compositions and in vitro antioxidant properties of aqueous leaf extract of Myrianthus arboreus plant. The phytochemical analysis was determined using standard laboratory methods and the in vitro antioxidant properties assessed by the 1,1-diphenyl-2- picrylhydrazyl (DPPH) free radical scavenging and Reducing Power assay. The leaf extract showed the presence of saponins, tannins, alkaloids, flavonoids, glycosides and total phenol. These phytochemicals are in varying concentrations: saponins (2.05±0.02mg/g), flavonoids (0.33 ± 0.02mg/g), alkaloids (0.02±0.00mg/g), tannins (5.32 ± 0.01mg/g), total phenol (11.02 ± 1.00mg/g) and glycosides (0.48 ± 0.00mg/g). Also, the result of the antioxidant studies revealed that the percentage inhibition of Myrianthus arboreus aqueous leaf extract against free radical at different concentrations of 125µg/ml, 100µg/ml, 75µg/ml, 50µg/ml and 25µg/ml for both assays gave 74.4% and 64.5% respectively as compared to the standard (Ascorbic acid) values of 101.0% and 94.1%. Thus, the antioxidant activity of the extract was significant (p<0.05) for both assays when compared with the standard. However, the Reducing Power assay revealed increased significance. This implies that the inhibitory concentration (IC⁵⁰) of Myrianthus arboreus leaf extract is lower than the standard ascorbic acid and therefore suggests that it is a potent antioxidant capable of scavenging excess free radicals. In conclusion, it can be deduced that the phytochemical compositions especially the total phenols which are higher in concentration when compared with other phytochemicals are responsible for the antioxidant properties of the plant and can invariably serve as natural supplements of antioxidant in foods and pharmaceutical products.

Published in	International Journal of Biomedical Science and Engineering (Volume 5, Issue 1)
DOI	10.11648/j.ijbse.20170501.12
Page(s)	9-13
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2017. Published by Science Publishing Group

Keywords

Antioxidant, Free Radicals, Myrianthus arboreus and Phytochemicals

References

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APA Style

Awodi Patience Ilecholubo, Abbah Christopher Okpachi, Ejembi Daniel Ocholi. (2017). Phytochemical Constituents and Antioxidant Properties of Aqueous Leaf Extract of Myrianthus arboreus. International Journal of Biomedical Science and Engineering, 5(1), 9-13. https://doi.org/10.11648/j.ijbse.20170501.12

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ACS Style

Awodi Patience Ilecholubo; Abbah Christopher Okpachi; Ejembi Daniel Ocholi. Phytochemical Constituents and Antioxidant Properties of Aqueous Leaf Extract of Myrianthus arboreus. Int. J. Biomed. Sci. Eng. 2017, 5(1), 9-13. doi: 10.11648/j.ijbse.20170501.12

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AMA Style

Awodi Patience Ilecholubo, Abbah Christopher Okpachi, Ejembi Daniel Ocholi. Phytochemical Constituents and Antioxidant Properties of Aqueous Leaf Extract of Myrianthus arboreus. Int J Biomed Sci Eng. 2017;5(1):9-13. doi: 10.11648/j.ijbse.20170501.12

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@article{10.11648/j.ijbse.20170501.12,
  author = {Awodi Patience Ilecholubo and Abbah Christopher Okpachi and Ejembi Daniel Ocholi},
  title = {Phytochemical Constituents and Antioxidant Properties of Aqueous Leaf Extract of Myrianthus arboreus},
  journal = {International Journal of Biomedical Science and Engineering},
  volume = {5},
  number = {1},
  pages = {9-13},
  doi = {10.11648/j.ijbse.20170501.12},
  url = {https://doi.org/10.11648/j.ijbse.20170501.12},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijbse.20170501.12},
  abstract = {This study was carried out to determine the phytochemical compositions and in vitro antioxidant properties of aqueous leaf extract of Myrianthus arboreus plant. The phytochemical analysis was determined using standard laboratory methods and the in vitro antioxidant properties assessed by the 1,1-diphenyl-2- picrylhydrazyl (DPPH) free radical scavenging and Reducing Power assay. The leaf extract showed the presence of saponins, tannins, alkaloids, flavonoids, glycosides and total phenol. These phytochemicals are in varying concentrations: saponins (2.05±0.02mg/g), flavonoids (0.33 ± 0.02mg/g), alkaloids (0.02±0.00mg/g), tannins (5.32 ± 0.01mg/g), total phenol (11.02 ± 1.00mg/g) and glycosides (0.48 ± 0.00mg/g). Also, the result of the antioxidant studies revealed that the percentage inhibition of Myrianthus arboreus aqueous leaf extract against free radical at different concentrations of 125µg/ml, 100µg/ml, 75µg/ml, 50µg/ml and 25µg/ml for both assays gave 74.4% and 64.5% respectively as compared to the standard (Ascorbic acid) values of 101.0% and 94.1%. Thus, the antioxidant activity of the extract was significant (p50) of Myrianthus arboreus leaf extract is lower than the standard ascorbic acid and therefore suggests that it is a potent antioxidant capable of scavenging excess free radicals. In conclusion, it can be deduced that the phytochemical compositions especially the total phenols which are higher in concentration when compared with other phytochemicals are responsible for the antioxidant properties of the plant and can invariably serve as natural supplements of antioxidant in foods and pharmaceutical products.},
 year = {2017}
}

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TY  - JOUR
T1  - Phytochemical Constituents and Antioxidant Properties of Aqueous Leaf Extract of Myrianthus arboreus
AU  - Awodi Patience Ilecholubo
AU  - Abbah Christopher Okpachi
AU  - Ejembi Daniel Ocholi
Y1  - 2017/02/22
PY  - 2017
N1  - https://doi.org/10.11648/j.ijbse.20170501.12
DO  - 10.11648/j.ijbse.20170501.12
T2  - International Journal of Biomedical Science and Engineering
JF  - International Journal of Biomedical Science and Engineering
JO  - International Journal of Biomedical Science and Engineering
SP  - 9
EP  - 13
PB  - Science Publishing Group
SN  - 2376-7235
UR  - https://doi.org/10.11648/j.ijbse.20170501.12
AB  - This study was carried out to determine the phytochemical compositions and in vitro antioxidant properties of aqueous leaf extract of Myrianthus arboreus plant. The phytochemical analysis was determined using standard laboratory methods and the in vitro antioxidant properties assessed by the 1,1-diphenyl-2- picrylhydrazyl (DPPH) free radical scavenging and Reducing Power assay. The leaf extract showed the presence of saponins, tannins, alkaloids, flavonoids, glycosides and total phenol. These phytochemicals are in varying concentrations: saponins (2.05±0.02mg/g), flavonoids (0.33 ± 0.02mg/g), alkaloids (0.02±0.00mg/g), tannins (5.32 ± 0.01mg/g), total phenol (11.02 ± 1.00mg/g) and glycosides (0.48 ± 0.00mg/g). Also, the result of the antioxidant studies revealed that the percentage inhibition of Myrianthus arboreus aqueous leaf extract against free radical at different concentrations of 125µg/ml, 100µg/ml, 75µg/ml, 50µg/ml and 25µg/ml for both assays gave 74.4% and 64.5% respectively as compared to the standard (Ascorbic acid) values of 101.0% and 94.1%. Thus, the antioxidant activity of the extract was significant (p50) of Myrianthus arboreus leaf extract is lower than the standard ascorbic acid and therefore suggests that it is a potent antioxidant capable of scavenging excess free radicals. In conclusion, it can be deduced that the phytochemical compositions especially the total phenols which are higher in concentration when compared with other phytochemicals are responsible for the antioxidant properties of the plant and can invariably serve as natural supplements of antioxidant in foods and pharmaceutical products.
VL  - 5
IS  - 1
ER  -

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Author Information

Awodi Patience Ilecholubo

Department of Biochemistry, Kogi State University, Anyigba, Nigeria
Abbah Christopher Okpachi

Department of Biochemistry, Kogi State University, Anyigba, Nigeria
Ejembi Daniel Ocholi

Department of Biochemistry, Kogi State University, Anyigba, Nigeria

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APA Style

Awodi Patience Ilecholubo, Abbah Christopher Okpachi, Ejembi Daniel Ocholi. (2017). Phytochemical Constituents and Antioxidant Properties of Aqueous Leaf Extract of Myrianthus arboreus. International Journal of Biomedical Science and Engineering, 5(1), 9-13. https://doi.org/10.11648/j.ijbse.20170501.12

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ACS Style

Awodi Patience Ilecholubo; Abbah Christopher Okpachi; Ejembi Daniel Ocholi. Phytochemical Constituents and Antioxidant Properties of Aqueous Leaf Extract of Myrianthus arboreus. Int. J. Biomed. Sci. Eng. 2017, 5(1), 9-13. doi: 10.11648/j.ijbse.20170501.12

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AMA Style

Awodi Patience Ilecholubo, Abbah Christopher Okpachi, Ejembi Daniel Ocholi. Phytochemical Constituents and Antioxidant Properties of Aqueous Leaf Extract of Myrianthus arboreus. Int J Biomed Sci Eng. 2017;5(1):9-13. doi: 10.11648/j.ijbse.20170501.12

Copy | Download

@article{10.11648/j.ijbse.20170501.12,
  author = {Awodi Patience Ilecholubo and Abbah Christopher Okpachi and Ejembi Daniel Ocholi},
  title = {Phytochemical Constituents and Antioxidant Properties of Aqueous Leaf Extract of Myrianthus arboreus},
  journal = {International Journal of Biomedical Science and Engineering},
  volume = {5},
  number = {1},
  pages = {9-13},
  doi = {10.11648/j.ijbse.20170501.12},
  url = {https://doi.org/10.11648/j.ijbse.20170501.12},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijbse.20170501.12},
  abstract = {This study was carried out to determine the phytochemical compositions and in vitro antioxidant properties of aqueous leaf extract of Myrianthus arboreus plant. The phytochemical analysis was determined using standard laboratory methods and the in vitro antioxidant properties assessed by the 1,1-diphenyl-2- picrylhydrazyl (DPPH) free radical scavenging and Reducing Power assay. The leaf extract showed the presence of saponins, tannins, alkaloids, flavonoids, glycosides and total phenol. These phytochemicals are in varying concentrations: saponins (2.05±0.02mg/g), flavonoids (0.33 ± 0.02mg/g), alkaloids (0.02±0.00mg/g), tannins (5.32 ± 0.01mg/g), total phenol (11.02 ± 1.00mg/g) and glycosides (0.48 ± 0.00mg/g). Also, the result of the antioxidant studies revealed that the percentage inhibition of Myrianthus arboreus aqueous leaf extract against free radical at different concentrations of 125µg/ml, 100µg/ml, 75µg/ml, 50µg/ml and 25µg/ml for both assays gave 74.4% and 64.5% respectively as compared to the standard (Ascorbic acid) values of 101.0% and 94.1%. Thus, the antioxidant activity of the extract was significant (p50) of Myrianthus arboreus leaf extract is lower than the standard ascorbic acid and therefore suggests that it is a potent antioxidant capable of scavenging excess free radicals. In conclusion, it can be deduced that the phytochemical compositions especially the total phenols which are higher in concentration when compared with other phytochemicals are responsible for the antioxidant properties of the plant and can invariably serve as natural supplements of antioxidant in foods and pharmaceutical products.},
 year = {2017}
}

Copy | Download

TY  - JOUR
T1  - Phytochemical Constituents and Antioxidant Properties of Aqueous Leaf Extract of Myrianthus arboreus
AU  - Awodi Patience Ilecholubo
AU  - Abbah Christopher Okpachi
AU  - Ejembi Daniel Ocholi
Y1  - 2017/02/22
PY  - 2017
N1  - https://doi.org/10.11648/j.ijbse.20170501.12
DO  - 10.11648/j.ijbse.20170501.12
T2  - International Journal of Biomedical Science and Engineering
JF  - International Journal of Biomedical Science and Engineering
JO  - International Journal of Biomedical Science and Engineering
SP  - 9
EP  - 13
PB  - Science Publishing Group
SN  - 2376-7235
UR  - https://doi.org/10.11648/j.ijbse.20170501.12
AB  - This study was carried out to determine the phytochemical compositions and in vitro antioxidant properties of aqueous leaf extract of Myrianthus arboreus plant. The phytochemical analysis was determined using standard laboratory methods and the in vitro antioxidant properties assessed by the 1,1-diphenyl-2- picrylhydrazyl (DPPH) free radical scavenging and Reducing Power assay. The leaf extract showed the presence of saponins, tannins, alkaloids, flavonoids, glycosides and total phenol. These phytochemicals are in varying concentrations: saponins (2.05±0.02mg/g), flavonoids (0.33 ± 0.02mg/g), alkaloids (0.02±0.00mg/g), tannins (5.32 ± 0.01mg/g), total phenol (11.02 ± 1.00mg/g) and glycosides (0.48 ± 0.00mg/g). Also, the result of the antioxidant studies revealed that the percentage inhibition of Myrianthus arboreus aqueous leaf extract against free radical at different concentrations of 125µg/ml, 100µg/ml, 75µg/ml, 50µg/ml and 25µg/ml for both assays gave 74.4% and 64.5% respectively as compared to the standard (Ascorbic acid) values of 101.0% and 94.1%. Thus, the antioxidant activity of the extract was significant (p50) of Myrianthus arboreus leaf extract is lower than the standard ascorbic acid and therefore suggests that it is a potent antioxidant capable of scavenging excess free radicals. In conclusion, it can be deduced that the phytochemical compositions especially the total phenols which are higher in concentration when compared with other phytochemicals are responsible for the antioxidant properties of the plant and can invariably serve as natural supplements of antioxidant in foods and pharmaceutical products.
VL  - 5
IS  - 1
ER  -

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