Background: In Ghana, the HIV-1 profile has been quite dynamic. Previous reports identified HIV-1 subtypes A, D and G present and recently the CRF02_AG has been described as the predominant molecular form of HIV-1 in Kumasi, Ghana. This underscores the need for constant molecular characterization of HIV-1 species in the country. Objective: To provide current updates on the nature of HIV subtypes in Ghana, there is need for a user-friendly tool for routine monitoring of subtypes in the absence of cost-intensive and skill-demanding sequencing techniques. This work demonstrates the use of heteroduplex mobility assay (HMA) for rapid subtype detection of HIV-1 isolated from Ghanaian patients. Method: Viruses from 15 antibody-positive HIV-1 patients were isolated directly by co-culturing peripheral blood mononuclear cells (PBMC) with phytohemagglutinin (PHA)-stimulated donor PBMCs from an HIV seronegative individual and through HeLa cells positive for CD4/CXCR4/CCR5 (MAGIC-5A cells). That was followed by proviral DNA extraction. Heteroduplex Mobility Assay (HMA) technique was then performed on the HIV gag gene. Results: Using the HMA technique, newly isolated HIV-1 strains were subtyped as follows: There were seven subtype A (47%), two subtype G (13%) and six (40%) A/G recombinants. Conclusion: The HIV-1 CRF02_AG in Ghana has spread much more rapidly than the previously predominant subtype A over the years. Constant molecular characterization of HIV strains is necessary to enable clear elucidation of the prevailing HIV species in Ghana. This study presents the HMA as a useful tool for monitoring subtype emergence and distribution in the country.
| Published in | International Journal of Biomedical Science and Engineering (Volume 2, Issue 4) |
| DOI | 10.11648/j.ijbse.20140204.12 |
| Page(s) | 33-37 |
| Creative Commons |
This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited. |
| Copyright |
Copyright © The Author(s), 2014. Published by Science Publishing Group |
HIV-1 Subtype, HMA, Ghana
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APA Style
Nicholas Israel Nii-Trebi, James Ashun Mensah Brandful, William Kwabena Ampofo, Billal Obeng Musah, Jacob Samson Barnor, et al. (2014). Rapid Detection of HIV-1 Subtypes in Ghana by Heteroduplex Mobility Assay. International Journal of Biomedical Science and Engineering, 2(4), 33-37. https://doi.org/10.11648/j.ijbse.20140204.12
ACS Style
Nicholas Israel Nii-Trebi; James Ashun Mensah Brandful; William Kwabena Ampofo; Billal Obeng Musah; Jacob Samson Barnor, et al. Rapid Detection of HIV-1 Subtypes in Ghana by Heteroduplex Mobility Assay. Int. J. Biomed. Sci. Eng. 2014, 2(4), 33-37. doi: 10.11648/j.ijbse.20140204.12
AMA Style
Nicholas Israel Nii-Trebi, James Ashun Mensah Brandful, William Kwabena Ampofo, Billal Obeng Musah, Jacob Samson Barnor, et al. Rapid Detection of HIV-1 Subtypes in Ghana by Heteroduplex Mobility Assay. Int J Biomed Sci Eng. 2014;2(4):33-37. doi: 10.11648/j.ijbse.20140204.12
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Download@article{10.11648/j.ijbse.20140204.12,
author = {Nicholas Israel Nii-Trebi and James Ashun Mensah Brandful and William Kwabena Ampofo and Billal Obeng Musah and Jacob Samson Barnor and Kenzo Tokunaga},
title = {Rapid Detection of HIV-1 Subtypes in Ghana by Heteroduplex Mobility Assay},
journal = {International Journal of Biomedical Science and Engineering},
volume = {2},
number = {4},
pages = {33-37},
doi = {10.11648/j.ijbse.20140204.12},
url = {https://doi.org/10.11648/j.ijbse.20140204.12},
eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijbse.20140204.12},
abstract = {Background: In Ghana, the HIV-1 profile has been quite dynamic. Previous reports identified HIV-1 subtypes A, D and G present and recently the CRF02_AG has been described as the predominant molecular form of HIV-1 in Kumasi, Ghana. This underscores the need for constant molecular characterization of HIV-1 species in the country. Objective: To provide current updates on the nature of HIV subtypes in Ghana, there is need for a user-friendly tool for routine monitoring of subtypes in the absence of cost-intensive and skill-demanding sequencing techniques. This work demonstrates the use of heteroduplex mobility assay (HMA) for rapid subtype detection of HIV-1 isolated from Ghanaian patients. Method: Viruses from 15 antibody-positive HIV-1 patients were isolated directly by co-culturing peripheral blood mononuclear cells (PBMC) with phytohemagglutinin (PHA)-stimulated donor PBMCs from an HIV seronegative individual and through HeLa cells positive for CD4/CXCR4/CCR5 (MAGIC-5A cells). That was followed by proviral DNA extraction. Heteroduplex Mobility Assay (HMA) technique was then performed on the HIV gag gene. Results: Using the HMA technique, newly isolated HIV-1 strains were subtyped as follows: There were seven subtype A (47%), two subtype G (13%) and six (40%) A/G recombinants. Conclusion: The HIV-1 CRF02_AG in Ghana has spread much more rapidly than the previously predominant subtype A over the years. Constant molecular characterization of HIV strains is necessary to enable clear elucidation of the prevailing HIV species in Ghana. This study presents the HMA as a useful tool for monitoring subtype emergence and distribution in the country.},
year = {2014}
}
TY - JOUR T1 - Rapid Detection of HIV-1 Subtypes in Ghana by Heteroduplex Mobility Assay AU - Nicholas Israel Nii-Trebi AU - James Ashun Mensah Brandful AU - William Kwabena Ampofo AU - Billal Obeng Musah AU - Jacob Samson Barnor AU - Kenzo Tokunaga Y1 - 2014/09/30 PY - 2014 N1 - https://doi.org/10.11648/j.ijbse.20140204.12 DO - 10.11648/j.ijbse.20140204.12 T2 - International Journal of Biomedical Science and Engineering JF - International Journal of Biomedical Science and Engineering JO - International Journal of Biomedical Science and Engineering SP - 33 EP - 37 PB - Science Publishing Group SN - 2376-7235 UR - https://doi.org/10.11648/j.ijbse.20140204.12 AB - Background: In Ghana, the HIV-1 profile has been quite dynamic. Previous reports identified HIV-1 subtypes A, D and G present and recently the CRF02_AG has been described as the predominant molecular form of HIV-1 in Kumasi, Ghana. This underscores the need for constant molecular characterization of HIV-1 species in the country. Objective: To provide current updates on the nature of HIV subtypes in Ghana, there is need for a user-friendly tool for routine monitoring of subtypes in the absence of cost-intensive and skill-demanding sequencing techniques. This work demonstrates the use of heteroduplex mobility assay (HMA) for rapid subtype detection of HIV-1 isolated from Ghanaian patients. Method: Viruses from 15 antibody-positive HIV-1 patients were isolated directly by co-culturing peripheral blood mononuclear cells (PBMC) with phytohemagglutinin (PHA)-stimulated donor PBMCs from an HIV seronegative individual and through HeLa cells positive for CD4/CXCR4/CCR5 (MAGIC-5A cells). That was followed by proviral DNA extraction. Heteroduplex Mobility Assay (HMA) technique was then performed on the HIV gag gene. Results: Using the HMA technique, newly isolated HIV-1 strains were subtyped as follows: There were seven subtype A (47%), two subtype G (13%) and six (40%) A/G recombinants. Conclusion: The HIV-1 CRF02_AG in Ghana has spread much more rapidly than the previously predominant subtype A over the years. Constant molecular characterization of HIV strains is necessary to enable clear elucidation of the prevailing HIV species in Ghana. This study presents the HMA as a useful tool for monitoring subtype emergence and distribution in the country. VL - 2 IS - 4 ER -
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