Invasive alien ants are one of the most aggressive, competitive, and widespread invasive alien species around the world. Wasmannia auropunctata, native to the neotropical zoogeographic area, has been inadvertently introduced all over the world. The first disclosed record of field establishment of W. auropunctata in Chinese mainland was reported in 2022. In this paper, we aim to establish a rapid detection method targeting the W. auropunctata polymorphic microsatellite locus Waur-275, based on the microfluidic chip technology, to contribute to an active response to the crisis caused by this invasive pest. Eight primer sets were generated using the PrimerExplorer v5 online service, and the fifth primer set selected had the lowest Ct in the volume ratio of 8 (FIP/BIP:F3/B3). Method validity with six W. auropunctata samples confirmed the efficiency of this assay. High specificity was demonstrated by the positive result for W. auropunctata out of 6 related insect samples tested. Sensitivity analysis showed that this microfluidic chip method could achieve the detection limit of 1.00×101 copies/μL. A rapid screening method is essential for the whole process of interception and control of W. auropunctata in China. It can also help determine the origin of invasion, clarify the path of introduction to ensure biosecurity, and also uncover other possible undetected establishments in the wild.
| Published in | American Journal of Bioscience and Bioengineering (Volume 12, Issue 1) |
| DOI | 10.11648/j.bio.20241201.13 |
| Page(s) | 12-23 |
| Creative Commons |
This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited. |
| Copyright |
Copyright © The Author(s), 2024. Published by Science Publishing Group |
Rapid Screening, Microfluidic Chip, Wasmannia auropunctata, Invasive Alien Ants
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APA Style
Jiaying, W., Li, L., Yuanjing, W., Junxia, C. (2024). Establishment of a Microfluidic Chip Based Rapid Detection Method for Wasmannia auropunctata. American Journal of Bioscience and Bioengineering, 12(1), 12-23. https://doi.org/10.11648/j.bio.20241201.13
ACS Style
Jiaying, W.; Li, L.; Yuanjing, W.; Junxia, C. Establishment of a Microfluidic Chip Based Rapid Detection Method for Wasmannia auropunctata. Am. J. BioSci. Bioeng. 2024, 12(1), 12-23. doi: 10.11648/j.bio.20241201.13
AMA Style
Jiaying W, Li L, Yuanjing W, Junxia C. Establishment of a Microfluidic Chip Based Rapid Detection Method for Wasmannia auropunctata. Am J BioSci Bioeng. 2024;12(1):12-23. doi: 10.11648/j.bio.20241201.13
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Download@article{10.11648/j.bio.20241201.13,
author = {Wang Jiaying and Liu Li and Wang Yuanjing and Cui Junxia},
title = {Establishment of a Microfluidic Chip Based Rapid Detection Method for Wasmannia auropunctata},
journal = {American Journal of Bioscience and Bioengineering},
volume = {12},
number = {1},
pages = {12-23},
doi = {10.11648/j.bio.20241201.13},
url = {https://doi.org/10.11648/j.bio.20241201.13},
eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.bio.20241201.13},
abstract = {Invasive alien ants are one of the most aggressive, competitive, and widespread invasive alien species around the world. Wasmannia auropunctata, native to the neotropical zoogeographic area, has been inadvertently introduced all over the world. The first disclosed record of field establishment of W. auropunctata in Chinese mainland was reported in 2022. In this paper, we aim to establish a rapid detection method targeting the W. auropunctata polymorphic microsatellite locus Waur-275, based on the microfluidic chip technology, to contribute to an active response to the crisis caused by this invasive pest. Eight primer sets were generated using the PrimerExplorer v5 online service, and the fifth primer set selected had the lowest Ct in the volume ratio of 8 (FIP/BIP:F3/B3). Method validity with six W. auropunctata samples confirmed the efficiency of this assay. High specificity was demonstrated by the positive result for W. auropunctata out of 6 related insect samples tested. Sensitivity analysis showed that this microfluidic chip method could achieve the detection limit of 1.00×101 copies/μL. A rapid screening method is essential for the whole process of interception and control of W. auropunctata in China. It can also help determine the origin of invasion, clarify the path of introduction to ensure biosecurity, and also uncover other possible undetected establishments in the wild.
},
year = {2024}
}
TY - JOUR T1 - Establishment of a Microfluidic Chip Based Rapid Detection Method for Wasmannia auropunctata AU - Wang Jiaying AU - Liu Li AU - Wang Yuanjing AU - Cui Junxia Y1 - 2024/02/21 PY - 2024 N1 - https://doi.org/10.11648/j.bio.20241201.13 DO - 10.11648/j.bio.20241201.13 T2 - American Journal of Bioscience and Bioengineering JF - American Journal of Bioscience and Bioengineering JO - American Journal of Bioscience and Bioengineering SP - 12 EP - 23 PB - Science Publishing Group SN - 2328-5893 UR - https://doi.org/10.11648/j.bio.20241201.13 AB - Invasive alien ants are one of the most aggressive, competitive, and widespread invasive alien species around the world. Wasmannia auropunctata, native to the neotropical zoogeographic area, has been inadvertently introduced all over the world. The first disclosed record of field establishment of W. auropunctata in Chinese mainland was reported in 2022. In this paper, we aim to establish a rapid detection method targeting the W. auropunctata polymorphic microsatellite locus Waur-275, based on the microfluidic chip technology, to contribute to an active response to the crisis caused by this invasive pest. Eight primer sets were generated using the PrimerExplorer v5 online service, and the fifth primer set selected had the lowest Ct in the volume ratio of 8 (FIP/BIP:F3/B3). Method validity with six W. auropunctata samples confirmed the efficiency of this assay. High specificity was demonstrated by the positive result for W. auropunctata out of 6 related insect samples tested. Sensitivity analysis showed that this microfluidic chip method could achieve the detection limit of 1.00×101 copies/μL. A rapid screening method is essential for the whole process of interception and control of W. auropunctata in China. It can also help determine the origin of invasion, clarify the path of introduction to ensure biosecurity, and also uncover other possible undetected establishments in the wild. VL - 12 IS - 1 ER -
Technical Center, Ningbo Customs, Ningbo, China; Ningbo Academy of Inspection and Quarantine, Ningbo, China
