Oleic Acid Produces Motor Incoordination and Hypoactivity in Infant Wistar Rats Through GABA<sub>A</sub> Receptors

Gabriel Guillén-Ruiz; Blandina Bernal-Morales; Carlos M. Contreras; Jonathan Cueto-Escobedo; Juan Francisco Rodríguez-Landa

doi:doi:10.11648/j.ajpn.20160402.11

Oleic Acid Produces Motor Incoordination and Hypoactivity in Infant Wistar Rats Through GABA_A Receptors

Gabriel Guillén-Ruiz, Blandina Bernal-Morales, Carlos M. Contreras, Jonathan Cueto-Escobedo, Juan Francisco Rodríguez-Landa

Published in American Journal of Psychiatry and Neuroscience (Volume 4, Issue 2)

Received: 8 March 2016 Accepted: 18 March 2016 Published: 31 March 2016

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Abstract

A mixture of eight fatty acids (linoleic, oleic, palmitic, stearic, myristic, elaidic, lauric, and palmitoleic acids) at similar concentrations that have been identified in human amniotic fluid exerts anxiolytic-like effects similar to diazepam in adult Wistar rats through actions at -aminobutyric acid-A (GABA_A) receptors, but unknown is whether any of these fatty acids exerts a predominant action over the others in infant rats. Of these fatty acids, some actions of oleic acid have already been identified, and it is one of the most abundant in amniotic fluid. Therefore, the aim of this study was to explore the effect of oleic acid on anxiety-like behavior and motoric activity in infant rats. To explore sedative actions, 28-day-old Wistar rats received 80-320 µg oleic acid or a sedative dose of diazepam (5 mg/kg). In a dose-response study, other groups of rats were injected with 10-80 µg oleic acid or 1 mg/kg diazepam. In an interaction study, rats that received oleic acid were pretreated with the GABA_A receptor antagonists picrotoxin or flumazenil to explore the participation of this receptor in the effects of oleic acid on behavior in the elevated plus maze, rotarod test, and open field test. Oleic acid produced sedative effects but did not exert any anxiolytic-like actions. Hypoactivity and motor incoordination that were induced by oleic acid were blocked by flumazenil and picrotoxin. In conclusion, oleic acid reduced locomotor activity and motor incoordination through actions at the GABA_A receptor.

Published in	American Journal of Psychiatry and Neuroscience (Volume 4, Issue 2)
DOI	10.11648/j.ajpn.20160402.11
Page(s)	18-25
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2016. Published by Science Publishing Group

Keywords

Hypoactivity, Motor Incoordination, Fatty Acids, Oleic Acid, Anxiety

References

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Gabriel Guillén-Ruiz, Blandina Bernal-Morales, Carlos M. Contreras, Jonathan Cueto-Escobedo, Juan Francisco Rodríguez-Landa. (2016). Oleic Acid Produces Motor Incoordination and Hypoactivity in Infant Wistar Rats Through GABAA Receptors. American Journal of Psychiatry and Neuroscience, 4(2), 18-25. https://doi.org/10.11648/j.ajpn.20160402.11

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Gabriel Guillén-Ruiz; Blandina Bernal-Morales; Carlos M. Contreras; Jonathan Cueto-Escobedo; Juan Francisco Rodríguez-Landa. Oleic Acid Produces Motor Incoordination and Hypoactivity in Infant Wistar Rats Through GABAA Receptors. Am. J. Psychiatry Neurosci. 2016, 4(2), 18-25. doi: 10.11648/j.ajpn.20160402.11

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AMA Style

Gabriel Guillén-Ruiz, Blandina Bernal-Morales, Carlos M. Contreras, Jonathan Cueto-Escobedo, Juan Francisco Rodríguez-Landa. Oleic Acid Produces Motor Incoordination and Hypoactivity in Infant Wistar Rats Through GABAA Receptors. Am J Psychiatry Neurosci. 2016;4(2):18-25. doi: 10.11648/j.ajpn.20160402.11

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@article{10.11648/j.ajpn.20160402.11,
  author = {Gabriel Guillén-Ruiz and Blandina Bernal-Morales and Carlos M. Contreras and Jonathan Cueto-Escobedo and Juan Francisco Rodríguez-Landa},
  title = {Oleic Acid Produces Motor Incoordination and Hypoactivity in Infant Wistar Rats Through GABAA Receptors},
  journal = {American Journal of Psychiatry and Neuroscience},
  volume = {4},
  number = {2},
  pages = {18-25},
  doi = {10.11648/j.ajpn.20160402.11},
  url = {https://doi.org/10.11648/j.ajpn.20160402.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajpn.20160402.11},
  abstract = {A mixture of eight fatty acids (linoleic, oleic, palmitic, stearic, myristic, elaidic, lauric, and palmitoleic acids) at similar concentrations that have been identified in human amniotic fluid exerts anxiolytic-like effects similar to diazepam in adult Wistar rats through actions at -aminobutyric acid-A (GABAA) receptors, but unknown is whether any of these fatty acids exerts a predominant action over the others in infant rats. Of these fatty acids, some actions of oleic acid have already been identified, and it is one of the most abundant in amniotic fluid. Therefore, the aim of this study was to explore the effect of oleic acid on anxiety-like behavior and motoric activity in infant rats. To explore sedative actions, 28-day-old Wistar rats received 80-320 µg oleic acid or a sedative dose of diazepam (5 mg/kg). In a dose-response study, other groups of rats were injected with 10-80 µg oleic acid or 1 mg/kg diazepam. In an interaction study, rats that received oleic acid were pretreated with the GABAA receptor antagonists picrotoxin or flumazenil to explore the participation of this receptor in the effects of oleic acid on behavior in the elevated plus maze, rotarod test, and open field test. Oleic acid produced sedative effects but did not exert any anxiolytic-like actions. Hypoactivity and motor incoordination that were induced by oleic acid were blocked by flumazenil and picrotoxin. In conclusion, oleic acid reduced locomotor activity and motor incoordination through actions at the GABAA receptor.},
 year = {2016}
}

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TY  - JOUR
T1  - Oleic Acid Produces Motor Incoordination and Hypoactivity in Infant Wistar Rats Through GABAA Receptors
AU  - Gabriel Guillén-Ruiz
AU  - Blandina Bernal-Morales
AU  - Carlos M. Contreras
AU  - Jonathan Cueto-Escobedo
AU  - Juan Francisco Rodríguez-Landa
Y1  - 2016/03/31
PY  - 2016
N1  - https://doi.org/10.11648/j.ajpn.20160402.11
DO  - 10.11648/j.ajpn.20160402.11
T2  - American Journal of Psychiatry and Neuroscience
JF  - American Journal of Psychiatry and Neuroscience
JO  - American Journal of Psychiatry and Neuroscience
SP  - 18
EP  - 25
PB  - Science Publishing Group
SN  - 2330-426X
UR  - https://doi.org/10.11648/j.ajpn.20160402.11
AB  - A mixture of eight fatty acids (linoleic, oleic, palmitic, stearic, myristic, elaidic, lauric, and palmitoleic acids) at similar concentrations that have been identified in human amniotic fluid exerts anxiolytic-like effects similar to diazepam in adult Wistar rats through actions at -aminobutyric acid-A (GABAA) receptors, but unknown is whether any of these fatty acids exerts a predominant action over the others in infant rats. Of these fatty acids, some actions of oleic acid have already been identified, and it is one of the most abundant in amniotic fluid. Therefore, the aim of this study was to explore the effect of oleic acid on anxiety-like behavior and motoric activity in infant rats. To explore sedative actions, 28-day-old Wistar rats received 80-320 µg oleic acid or a sedative dose of diazepam (5 mg/kg). In a dose-response study, other groups of rats were injected with 10-80 µg oleic acid or 1 mg/kg diazepam. In an interaction study, rats that received oleic acid were pretreated with the GABAA receptor antagonists picrotoxin or flumazenil to explore the participation of this receptor in the effects of oleic acid on behavior in the elevated plus maze, rotarod test, and open field test. Oleic acid produced sedative effects but did not exert any anxiolytic-like actions. Hypoactivity and motor incoordination that were induced by oleic acid were blocked by flumazenil and picrotoxin. In conclusion, oleic acid reduced locomotor activity and motor incoordination through actions at the GABAA receptor.
VL  - 4
IS  - 2
ER  -

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Author Information

Gabriel Guillén-Ruiz

Neuropharmacology Section, Institute of Neuroethology, University of Veracruz, Xalapa, Veracruz, México
Blandina Bernal-Morales

Neuropharmacology Section, Institute of Neuroethology, University of Veracruz, Xalapa, Veracruz, México
Carlos M. Contreras

Neuropharmacology Section, Institute of Neuroethology, University of Veracruz, Xalapa, Veracruz, México
Jonathan Cueto-Escobedo

Neuropharmacology Section, Institute of Neuroethology, University of Veracruz, Xalapa, Veracruz, México
Juan Francisco Rodríguez-Landa

Neuropharmacology Section, Institute of Neuroethology, University of Veracruz, Xalapa, Veracruz, México

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Gabriel Guillén-Ruiz, Blandina Bernal-Morales, Carlos M. Contreras, Jonathan Cueto-Escobedo, Juan Francisco Rodríguez-Landa. (2016). Oleic Acid Produces Motor Incoordination and Hypoactivity in Infant Wistar Rats Through GABAA Receptors. American Journal of Psychiatry and Neuroscience, 4(2), 18-25. https://doi.org/10.11648/j.ajpn.20160402.11

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ACS Style

Gabriel Guillén-Ruiz; Blandina Bernal-Morales; Carlos M. Contreras; Jonathan Cueto-Escobedo; Juan Francisco Rodríguez-Landa. Oleic Acid Produces Motor Incoordination and Hypoactivity in Infant Wistar Rats Through GABAA Receptors. Am. J. Psychiatry Neurosci. 2016, 4(2), 18-25. doi: 10.11648/j.ajpn.20160402.11

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AMA Style

Gabriel Guillén-Ruiz, Blandina Bernal-Morales, Carlos M. Contreras, Jonathan Cueto-Escobedo, Juan Francisco Rodríguez-Landa. Oleic Acid Produces Motor Incoordination and Hypoactivity in Infant Wistar Rats Through GABAA Receptors. Am J Psychiatry Neurosci. 2016;4(2):18-25. doi: 10.11648/j.ajpn.20160402.11

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@article{10.11648/j.ajpn.20160402.11,
  author = {Gabriel Guillén-Ruiz and Blandina Bernal-Morales and Carlos M. Contreras and Jonathan Cueto-Escobedo and Juan Francisco Rodríguez-Landa},
  title = {Oleic Acid Produces Motor Incoordination and Hypoactivity in Infant Wistar Rats Through GABAA Receptors},
  journal = {American Journal of Psychiatry and Neuroscience},
  volume = {4},
  number = {2},
  pages = {18-25},
  doi = {10.11648/j.ajpn.20160402.11},
  url = {https://doi.org/10.11648/j.ajpn.20160402.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajpn.20160402.11},
  abstract = {A mixture of eight fatty acids (linoleic, oleic, palmitic, stearic, myristic, elaidic, lauric, and palmitoleic acids) at similar concentrations that have been identified in human amniotic fluid exerts anxiolytic-like effects similar to diazepam in adult Wistar rats through actions at -aminobutyric acid-A (GABAA) receptors, but unknown is whether any of these fatty acids exerts a predominant action over the others in infant rats. Of these fatty acids, some actions of oleic acid have already been identified, and it is one of the most abundant in amniotic fluid. Therefore, the aim of this study was to explore the effect of oleic acid on anxiety-like behavior and motoric activity in infant rats. To explore sedative actions, 28-day-old Wistar rats received 80-320 µg oleic acid or a sedative dose of diazepam (5 mg/kg). In a dose-response study, other groups of rats were injected with 10-80 µg oleic acid or 1 mg/kg diazepam. In an interaction study, rats that received oleic acid were pretreated with the GABAA receptor antagonists picrotoxin or flumazenil to explore the participation of this receptor in the effects of oleic acid on behavior in the elevated plus maze, rotarod test, and open field test. Oleic acid produced sedative effects but did not exert any anxiolytic-like actions. Hypoactivity and motor incoordination that were induced by oleic acid were blocked by flumazenil and picrotoxin. In conclusion, oleic acid reduced locomotor activity and motor incoordination through actions at the GABAA receptor.},
 year = {2016}
}

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TY  - JOUR
T1  - Oleic Acid Produces Motor Incoordination and Hypoactivity in Infant Wistar Rats Through GABAA Receptors
AU  - Gabriel Guillén-Ruiz
AU  - Blandina Bernal-Morales
AU  - Carlos M. Contreras
AU  - Jonathan Cueto-Escobedo
AU  - Juan Francisco Rodríguez-Landa
Y1  - 2016/03/31
PY  - 2016
N1  - https://doi.org/10.11648/j.ajpn.20160402.11
DO  - 10.11648/j.ajpn.20160402.11
T2  - American Journal of Psychiatry and Neuroscience
JF  - American Journal of Psychiatry and Neuroscience
JO  - American Journal of Psychiatry and Neuroscience
SP  - 18
EP  - 25
PB  - Science Publishing Group
SN  - 2330-426X
UR  - https://doi.org/10.11648/j.ajpn.20160402.11
AB  - A mixture of eight fatty acids (linoleic, oleic, palmitic, stearic, myristic, elaidic, lauric, and palmitoleic acids) at similar concentrations that have been identified in human amniotic fluid exerts anxiolytic-like effects similar to diazepam in adult Wistar rats through actions at -aminobutyric acid-A (GABAA) receptors, but unknown is whether any of these fatty acids exerts a predominant action over the others in infant rats. Of these fatty acids, some actions of oleic acid have already been identified, and it is one of the most abundant in amniotic fluid. Therefore, the aim of this study was to explore the effect of oleic acid on anxiety-like behavior and motoric activity in infant rats. To explore sedative actions, 28-day-old Wistar rats received 80-320 µg oleic acid or a sedative dose of diazepam (5 mg/kg). In a dose-response study, other groups of rats were injected with 10-80 µg oleic acid or 1 mg/kg diazepam. In an interaction study, rats that received oleic acid were pretreated with the GABAA receptor antagonists picrotoxin or flumazenil to explore the participation of this receptor in the effects of oleic acid on behavior in the elevated plus maze, rotarod test, and open field test. Oleic acid produced sedative effects but did not exert any anxiolytic-like actions. Hypoactivity and motor incoordination that were induced by oleic acid were blocked by flumazenil and picrotoxin. In conclusion, oleic acid reduced locomotor activity and motor incoordination through actions at the GABAA receptor.
VL  - 4
IS  - 2
ER  -

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