Germination in Vitro of Brassicaceae (Sinapis arvensis L.) in the Northern Region of Tlemcen (Algeria)

Benabadji Noury; Belkhodja Amal Nesrine; Abdeljalil Anissa

doi:doi:10.11648/j.ajls.20150304.15

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Germination in Vitro of Brassicaceae (Sinapis arvensis L.) in the Northern Region of Tlemcen (Algeria)

Benabadji Noury, Belkhodja Amal Nesrine, Abdeljalil Anissa

Published in American Journal of Life Sciences (Volume 3, Issue 4)

Received: 3 June 2015 Accepted: 17 June 2015 Published: 4 July 2015

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Abstract

This ecophysiological study was conducted on a species (Sinapis arvensis L.) widespread in nature in Algeria particularly in the province of Tlemcen. This plant, even if it is undesirable in crop fields, can be useful in maintaining the biodiversity of the plant world. The goal set in this study was to treat the micro-propagation and germination of the species Sinapsis arvensis L. in synthetic environments, since these phenomena of growth and development in a sterile environment are poorly controlled. Our work deals successively with the following results: - The in vitro germination of Sinapis arvensisin different synthetic media, seeds taken of pods are harvested in stations in the Tlemcen region (Zenata, Beni-Ghanam and Rachgoun stations).They germinate differently; the germination rates vary with temperature and media used (Nutrient Agar and Potatoes Dextrose Agar, distilled water with NaCl at different concentrations, 1 g/L, 3 g/L and 5 g/L). This phenological phase appears to respond positively to the experimentation multiple conditions. - The percentage of germination was 75%. - Contamination by pathogens reaches 25%, despite the taken precautions (sterility of plant material, cleaning glassware, etc.).

Published in	American Journal of Life Sciences (Volume 3, Issue 4)
DOI	10.11648/j.ajls.20150304.15
Page(s)	279-289
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2015. Published by Science Publishing Group

Keywords

Sinapis arvensis L., Nutrient Agar, Potatoes Dextrose Agar Medium, Germination, Region of Tlemcen, Algeria

References

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[2]	Augé R., Beauchesne G., Boccon-Gibod J., Decourtye L., Digat B., Jalouzot R., Minier R., Morand J .,Reynoird J. et Strullu D.,1989 - La culture in vitro et ses applications horticoles. 3ème édition revue, corrigée et augmentée. Ed Tech. Doc. Lavoisier 225 p.
[3]	Benchenfi Lachachi S., Benabadji N., Benmansour D., 2013 – Contribution to the study of Lygeum spartum L. germinative hospeties in the south region of Tlemcen (Western Algeria).Environment Reserch Journal 7(2) ISSN 1994-5396: 20-24.
[4]	Boccon-Gibod J., 1984 - Régénération du Crosne du Japon (Stachys sieboldii Mig.) par culture de méristème : multiplication et conservation in vitro des clones. In : Congrès sur l’application de la culture in vitro à l’amélioration desplantes potagères. EUCARPIA, section légumes, Versailles. 31- 41.
[5]	Dubey P.S and Mall L.P., 1972 – Ecology of germination of weed seeds. I. Role of temperature and depth of burial in soi. Oecologia, 10: 105-110.
[6]	Haicour R., 2002 - Biotechnologie végétale : technique de laboratoire. Ed Tec et Doc. Montréal. AUF, 2002 (université francophones ISBN 2-7430-0560-2). 275p.
[7]	Haines H., 1995 - The ecology of running waters. Liverpool University Press, Liverpool. 555p.
[8]	Harper J.L and Benton R.A., 1966 – The behavior of seeds in soil. II. The germination of seeds on the surface of water supplying substrate. J. Ecol. 54: 151-166.
[9]	Hebert Y., Lefort-Buson M. et Damaerval M., 1993 - Les outils d’évaluation de la diversité génétique. Agronomie. 8(3): 173-178.
[10]	Heller, 1990 - Physiologie végétale et développement 4ème Éd. Mass. Et Cie, 270p.
[11]	Jay Allmand C., Capelli P. and CornuD., 1992 - Root development of in vitro hybrid walnut microcutting in vermiculite containing gelrite medium. Station d’amélioration des arbres forestiers. INRA, 45160. Ardon France. ScienCIA horticultura. 51(3-4) : 335-342.
[12]	Jay Allmand C. et Capelli P., 1997-La multiplication végétative in-vitro, base méthodologique. D. E. A. Ressources génétiques et Amélioration des plantes. INA Paris Grignon. 101p.
[13]	Koller D and Hadas Y, 1982 – Water relations in the germination of seeds. In: Lange O.I., Osmond C.B and Ziegler H (Eds.) Encyclopedia of plant physiology. Springer-Verlag, Berlin. 12(B): 401-431.
[14]	Loukidi N., 1998 - Contribution à une étude morpho-histométrique de Malva sylvestris L. dans la région de Tlemcen. Mém. DES Physio. Vég. Univ. Tlemcen, 120p.
[15]	Margara F., 1989 - Bases de multiplication végétative : les méristèmes et l’organogénèse. Ed INRA, Paris.262p.
[16]	Ungar I. A., 1978– Halophyte seed germination. Bot. Rev., 44.233-264.
[17]	Van Der Toorn J. and Ten Hove H. J., 1982 – On the ecology of Cotula coronopifolia L. and Ranunculus sceleratus L. II. Experiments on germination, seed longevity, and seedling survival. Acta ɶcologica. ɶcol.Plant.Vol. 3 (17), n°4. 409-418.

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APA Style

Benabadji Noury, Belkhodja Amal Nesrine, Abdeljalil Anissa. (2015). Germination in Vitro of Brassicaceae (Sinapis arvensis L.) in the Northern Region of Tlemcen (Algeria). American Journal of Life Sciences, 3(4), 279-289. https://doi.org/10.11648/j.ajls.20150304.15

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ACS Style

Benabadji Noury; Belkhodja Amal Nesrine; Abdeljalil Anissa. Germination in Vitro of Brassicaceae (Sinapis arvensis L.) in the Northern Region of Tlemcen (Algeria). Am. J. Life Sci. 2015, 3(4), 279-289. doi: 10.11648/j.ajls.20150304.15

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AMA Style

Benabadji Noury, Belkhodja Amal Nesrine, Abdeljalil Anissa. Germination in Vitro of Brassicaceae (Sinapis arvensis L.) in the Northern Region of Tlemcen (Algeria). Am J Life Sci. 2015;3(4):279-289. doi: 10.11648/j.ajls.20150304.15

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@article{10.11648/j.ajls.20150304.15,
  author = {Benabadji Noury and Belkhodja Amal Nesrine and Abdeljalil Anissa},
  title = {Germination in Vitro of Brassicaceae (Sinapis arvensis L.) in the Northern Region of Tlemcen (Algeria)},
  journal = {American Journal of Life Sciences},
  volume = {3},
  number = {4},
  pages = {279-289},
  doi = {10.11648/j.ajls.20150304.15},
  url = {https://doi.org/10.11648/j.ajls.20150304.15},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajls.20150304.15},
  abstract = {This ecophysiological study was conducted on a species (Sinapis arvensis L.) widespread in nature in Algeria particularly in the province of Tlemcen. This plant, even if it is undesirable in crop fields, can be useful in maintaining the biodiversity of the plant world. The goal set in this study was to treat the micro-propagation and germination of the species Sinapsis arvensis L. in synthetic environments, since these phenomena of growth and development in a sterile environment are poorly controlled. Our work deals successively with the following results: - The in vitro germination of Sinapis arvensisin different synthetic media, seeds taken of pods are harvested in stations in the Tlemcen region (Zenata, Beni-Ghanam and Rachgoun stations).They germinate differently; the germination rates vary with temperature and media used (Nutrient Agar and Potatoes Dextrose Agar, distilled water with NaCl at different concentrations, 1 g/L, 3 g/L and 5 g/L). This phenological phase appears to respond positively to the experimentation multiple conditions. - The percentage of germination was 75%. - Contamination by pathogens reaches 25%, despite the taken precautions (sterility of plant material, cleaning glassware, etc.).},
 year = {2015}
}

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TY  - JOUR
T1  - Germination in Vitro of Brassicaceae (Sinapis arvensis L.) in the Northern Region of Tlemcen (Algeria)
AU  - Benabadji Noury
AU  - Belkhodja Amal Nesrine
AU  - Abdeljalil Anissa
Y1  - 2015/07/04
PY  - 2015
N1  - https://doi.org/10.11648/j.ajls.20150304.15
DO  - 10.11648/j.ajls.20150304.15
T2  - American Journal of Life Sciences
JF  - American Journal of Life Sciences
JO  - American Journal of Life Sciences
SP  - 279
EP  - 289
PB  - Science Publishing Group
SN  - 2328-5737
UR  - https://doi.org/10.11648/j.ajls.20150304.15
AB  - This ecophysiological study was conducted on a species (Sinapis arvensis L.) widespread in nature in Algeria particularly in the province of Tlemcen. This plant, even if it is undesirable in crop fields, can be useful in maintaining the biodiversity of the plant world. The goal set in this study was to treat the micro-propagation and germination of the species Sinapsis arvensis L. in synthetic environments, since these phenomena of growth and development in a sterile environment are poorly controlled. Our work deals successively with the following results: - The in vitro germination of Sinapis arvensisin different synthetic media, seeds taken of pods are harvested in stations in the Tlemcen region (Zenata, Beni-Ghanam and Rachgoun stations).They germinate differently; the germination rates vary with temperature and media used (Nutrient Agar and Potatoes Dextrose Agar, distilled water with NaCl at different concentrations, 1 g/L, 3 g/L and 5 g/L). This phenological phase appears to respond positively to the experimentation multiple conditions. - The percentage of germination was 75%. - Contamination by pathogens reaches 25%, despite the taken precautions (sterility of plant material, cleaning glassware, etc.).
VL  - 3
IS  - 4
ER  -

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Author Information

Benabadji Noury

Laboratory of Ecology and Management of the Natural Ecosystems, Department of Ecology and Environment, Aboubakr Belkaid University, Tlemcen, Algeria
Belkhodja Amal Nesrine

Laboratory of Ecology and Management of the Natural Ecosystems, Department of Ecology and Environment, Aboubakr Belkaid University, Tlemcen, Algeria
Abdeljalil Anissa

Laboratory of Ecology and Management of the Natural Ecosystems, Department of Ecology and Environment, Aboubakr Belkaid University, Tlemcen, Algeria

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APA Style

Benabadji Noury, Belkhodja Amal Nesrine, Abdeljalil Anissa. (2015). Germination in Vitro of Brassicaceae (Sinapis arvensis L.) in the Northern Region of Tlemcen (Algeria). American Journal of Life Sciences, 3(4), 279-289. https://doi.org/10.11648/j.ajls.20150304.15

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ACS Style

Benabadji Noury; Belkhodja Amal Nesrine; Abdeljalil Anissa. Germination in Vitro of Brassicaceae (Sinapis arvensis L.) in the Northern Region of Tlemcen (Algeria). Am. J. Life Sci. 2015, 3(4), 279-289. doi: 10.11648/j.ajls.20150304.15

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AMA Style

Benabadji Noury, Belkhodja Amal Nesrine, Abdeljalil Anissa. Germination in Vitro of Brassicaceae (Sinapis arvensis L.) in the Northern Region of Tlemcen (Algeria). Am J Life Sci. 2015;3(4):279-289. doi: 10.11648/j.ajls.20150304.15

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@article{10.11648/j.ajls.20150304.15,
  author = {Benabadji Noury and Belkhodja Amal Nesrine and Abdeljalil Anissa},
  title = {Germination in Vitro of Brassicaceae (Sinapis arvensis L.) in the Northern Region of Tlemcen (Algeria)},
  journal = {American Journal of Life Sciences},
  volume = {3},
  number = {4},
  pages = {279-289},
  doi = {10.11648/j.ajls.20150304.15},
  url = {https://doi.org/10.11648/j.ajls.20150304.15},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajls.20150304.15},
  abstract = {This ecophysiological study was conducted on a species (Sinapis arvensis L.) widespread in nature in Algeria particularly in the province of Tlemcen. This plant, even if it is undesirable in crop fields, can be useful in maintaining the biodiversity of the plant world. The goal set in this study was to treat the micro-propagation and germination of the species Sinapsis arvensis L. in synthetic environments, since these phenomena of growth and development in a sterile environment are poorly controlled. Our work deals successively with the following results: - The in vitro germination of Sinapis arvensisin different synthetic media, seeds taken of pods are harvested in stations in the Tlemcen region (Zenata, Beni-Ghanam and Rachgoun stations).They germinate differently; the germination rates vary with temperature and media used (Nutrient Agar and Potatoes Dextrose Agar, distilled water with NaCl at different concentrations, 1 g/L, 3 g/L and 5 g/L). This phenological phase appears to respond positively to the experimentation multiple conditions. - The percentage of germination was 75%. - Contamination by pathogens reaches 25%, despite the taken precautions (sterility of plant material, cleaning glassware, etc.).},
 year = {2015}
}

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TY  - JOUR
T1  - Germination in Vitro of Brassicaceae (Sinapis arvensis L.) in the Northern Region of Tlemcen (Algeria)
AU  - Benabadji Noury
AU  - Belkhodja Amal Nesrine
AU  - Abdeljalil Anissa
Y1  - 2015/07/04
PY  - 2015
N1  - https://doi.org/10.11648/j.ajls.20150304.15
DO  - 10.11648/j.ajls.20150304.15
T2  - American Journal of Life Sciences
JF  - American Journal of Life Sciences
JO  - American Journal of Life Sciences
SP  - 279
EP  - 289
PB  - Science Publishing Group
SN  - 2328-5737
UR  - https://doi.org/10.11648/j.ajls.20150304.15
AB  - This ecophysiological study was conducted on a species (Sinapis arvensis L.) widespread in nature in Algeria particularly in the province of Tlemcen. This plant, even if it is undesirable in crop fields, can be useful in maintaining the biodiversity of the plant world. The goal set in this study was to treat the micro-propagation and germination of the species Sinapsis arvensis L. in synthetic environments, since these phenomena of growth and development in a sterile environment are poorly controlled. Our work deals successively with the following results: - The in vitro germination of Sinapis arvensisin different synthetic media, seeds taken of pods are harvested in stations in the Tlemcen region (Zenata, Beni-Ghanam and Rachgoun stations).They germinate differently; the germination rates vary with temperature and media used (Nutrient Agar and Potatoes Dextrose Agar, distilled water with NaCl at different concentrations, 1 g/L, 3 g/L and 5 g/L). This phenological phase appears to respond positively to the experimentation multiple conditions. - The percentage of germination was 75%. - Contamination by pathogens reaches 25%, despite the taken precautions (sterility of plant material, cleaning glassware, etc.).
VL  - 3
IS  - 4
ER  -

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