Cellulase are the enzymes hydrolyzing cellulosic biomass and are produced by the microorganism that grown over cellulosic matter. Bacterial Cellulases poses more advantage when compared to the cellulases from other sources. This study aims to determine the optimum conditions of cellulase production from the bacteria Bacillus Circulans CC2 and CC4 in comparison with isolates hydrolize rice straw. CC2 and CC4 isolated from larvae Cossus cossus. The testing to produce cellulase was done with various pH and temperature. The enzyme activity was tested using DNS Method. The results showed that the isolated CC2 and CC4 have the same optimum temperature of 70C on condition CMC 1%, medium pH 7,0. Crude extract cellulase isolates CC2 work optimally at pH 4 while the isolates CC4 work at pH 7. highest activity in the cellulase enzymes hydrolyze the substrate on cellulose powder with optimum activity at pH 4 CC4 isolates of 2.775 x 10-2 U/ mL, while the enzyme activity CC2 isolates 4.359 x 10-2 U/mL, p this occurs due to the enzymatic hydrolysis of cellulosic substrates with cellulase enzymes will outline the cellulose into glucose which is a simple form.
| Published in | American Journal of Biomedical and Life Sciences (Volume 4, Issue 2) |
| DOI | 10.11648/j.ajbls.20160402.13 |
| Page(s) | 21-25 |
| Creative Commons |
This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited. |
| Copyright |
Copyright © The Author(s), 2016. Published by Science Publishing Group |
Cellulace, Cossus Cossus, Rice Straw
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APA Style
Maswati Baharuddin, Ahyar Ahmad, Nursiah La Nafie, Firdaus Zenta. (2016). Cellulase Enzyme Activity of Bacillus Circulans from Larvae Cossus Cossus in Lignocellulosic Substrat. American Journal of Biomedical and Life Sciences, 4(2), 21-25. https://doi.org/10.11648/j.ajbls.20160402.13
ACS Style
Maswati Baharuddin; Ahyar Ahmad; Nursiah La Nafie; Firdaus Zenta. Cellulase Enzyme Activity of Bacillus Circulans from Larvae Cossus Cossus in Lignocellulosic Substrat. Am. J. Biomed. Life Sci. 2016, 4(2), 21-25. doi: 10.11648/j.ajbls.20160402.13
AMA Style
Maswati Baharuddin, Ahyar Ahmad, Nursiah La Nafie, Firdaus Zenta. Cellulase Enzyme Activity of Bacillus Circulans from Larvae Cossus Cossus in Lignocellulosic Substrat. Am J Biomed Life Sci. 2016;4(2):21-25. doi: 10.11648/j.ajbls.20160402.13
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Download@article{10.11648/j.ajbls.20160402.13,
author = {Maswati Baharuddin and Ahyar Ahmad and Nursiah La Nafie and Firdaus Zenta},
title = {Cellulase Enzyme Activity of Bacillus Circulans from Larvae Cossus Cossus in Lignocellulosic Substrat},
journal = {American Journal of Biomedical and Life Sciences},
volume = {4},
number = {2},
pages = {21-25},
doi = {10.11648/j.ajbls.20160402.13},
url = {https://doi.org/10.11648/j.ajbls.20160402.13},
eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajbls.20160402.13},
abstract = {Cellulase are the enzymes hydrolyzing cellulosic biomass and are produced by the microorganism that grown over cellulosic matter. Bacterial Cellulases poses more advantage when compared to the cellulases from other sources. This study aims to determine the optimum conditions of cellulase production from the bacteria Bacillus Circulans CC2 and CC4 in comparison with isolates hydrolize rice straw. CC2 and CC4 isolated from larvae Cossus cossus. The testing to produce cellulase was done with various pH and temperature. The enzyme activity was tested using DNS Method. The results showed that the isolated CC2 and CC4 have the same optimum temperature of 70C on condition CMC 1%, medium pH 7,0. Crude extract cellulase isolates CC2 work optimally at pH 4 while the isolates CC4 work at pH 7. highest activity in the cellulase enzymes hydrolyze the substrate on cellulose powder with optimum activity at pH 4 CC4 isolates of 2.775 x 10-2 U/ mL, while the enzyme activity CC2 isolates 4.359 x 10-2 U/mL, p this occurs due to the enzymatic hydrolysis of cellulosic substrates with cellulase enzymes will outline the cellulose into glucose which is a simple form.},
year = {2016}
}
TY - JOUR T1 - Cellulase Enzyme Activity of Bacillus Circulans from Larvae Cossus Cossus in Lignocellulosic Substrat AU - Maswati Baharuddin AU - Ahyar Ahmad AU - Nursiah La Nafie AU - Firdaus Zenta Y1 - 2016/03/28 PY - 2016 N1 - https://doi.org/10.11648/j.ajbls.20160402.13 DO - 10.11648/j.ajbls.20160402.13 T2 - American Journal of Biomedical and Life Sciences JF - American Journal of Biomedical and Life Sciences JO - American Journal of Biomedical and Life Sciences SP - 21 EP - 25 PB - Science Publishing Group SN - 2330-880X UR - https://doi.org/10.11648/j.ajbls.20160402.13 AB - Cellulase are the enzymes hydrolyzing cellulosic biomass and are produced by the microorganism that grown over cellulosic matter. Bacterial Cellulases poses more advantage when compared to the cellulases from other sources. This study aims to determine the optimum conditions of cellulase production from the bacteria Bacillus Circulans CC2 and CC4 in comparison with isolates hydrolize rice straw. CC2 and CC4 isolated from larvae Cossus cossus. The testing to produce cellulase was done with various pH and temperature. The enzyme activity was tested using DNS Method. The results showed that the isolated CC2 and CC4 have the same optimum temperature of 70C on condition CMC 1%, medium pH 7,0. Crude extract cellulase isolates CC2 work optimally at pH 4 while the isolates CC4 work at pH 7. highest activity in the cellulase enzymes hydrolyze the substrate on cellulose powder with optimum activity at pH 4 CC4 isolates of 2.775 x 10-2 U/ mL, while the enzyme activity CC2 isolates 4.359 x 10-2 U/mL, p this occurs due to the enzymatic hydrolysis of cellulosic substrates with cellulase enzymes will outline the cellulose into glucose which is a simple form. VL - 4 IS - 2 ER -
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