Splenic Niche Cells from Young Heterochronic Parabionts Have Decreased Capability to Amplify T-cell Proliferation in Vitro

Dmytro W. Shytikov; Maryna S. Shkumat; Tetiana M. Yankova; Alex G. Peregudov; Igor V. Artyuhov; Iryna M. Pishel

doi:doi:10.11648/j.ajbio.20150302.14

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Splenic Niche Cells from Young Heterochronic Parabionts Have Decreased Capability to Amplify T-cell Proliferation in Vitro

Dmytro W. Shytikov, Maryna S. Shkumat, Tetiana M. Yankova, Alex G. Peregudov, Igor V. Artyuhov, Iryna M. Pishel

Published in American Journal of BioScience (Volume 3, Issue 2)

Received: 1 February 2015 Accepted: 13 February 2015 Published: 2 March 2015

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Abstract

Immune system dysfunction during aging is well documented fact. Changes in the adaptive immune system are the most marked, especially in the T-cell compartment. In multiple preliminary studies it has been shown that parabiosis between 2 animals of different age can induce age-related changes in adaptive immune response and T-cell subpopulation composition of younger animal. In present study we evaluated the age-related changes in functions of rapidly renewing (macrophages) and slowly renewing cells (CD11c+ DCs) cells in spleens of young and old parabionts. We observed impaired capacity of splenic adherent cells from younger heterochronic parabionts to co-stimulate proliferation of autologous T-cells in vitro up to the level of old animals. Also we observed negative effect of this splenic cell population on intracellular signaling mechanisms that regulate PHA-activated proliferation of T-cells from young animals: statistically significant decrease of NFκB p65 expression and increased expression of IκBα during early activation events. This fact suggests that splenic macrophages may be involved in the induction of age-related changes of the immune system and they can be prospective target for further investigation.

Published in	American Journal of BioScience (Volume 3, Issue 2)
DOI	10.11648/j.ajbio.20150302.14
Page(s)	46-54
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2015. Published by Science Publishing Group

Keywords

CD11c+ Dendritic Cells, Macrophages, Heterochronic Parabiosis, Lymphoid Niche, Aging

References

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APA Style

Dmytro W. Shytikov, Maryna S. Shkumat, Tetiana M. Yankova, Alex G. Peregudov, Igor V. Artyuhov, et al. (2015). Splenic Niche Cells from Young Heterochronic Parabionts Have Decreased Capability to Amplify T-cell Proliferation in Vitro. American Journal of BioScience, 3(2), 46-54. https://doi.org/10.11648/j.ajbio.20150302.14

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ACS Style

Dmytro W. Shytikov; Maryna S. Shkumat; Tetiana M. Yankova; Alex G. Peregudov; Igor V. Artyuhov, et al. Splenic Niche Cells from Young Heterochronic Parabionts Have Decreased Capability to Amplify T-cell Proliferation in Vitro. Am. J. BioScience 2015, 3(2), 46-54. doi: 10.11648/j.ajbio.20150302.14

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AMA Style

Dmytro W. Shytikov, Maryna S. Shkumat, Tetiana M. Yankova, Alex G. Peregudov, Igor V. Artyuhov, et al. Splenic Niche Cells from Young Heterochronic Parabionts Have Decreased Capability to Amplify T-cell Proliferation in Vitro. Am J BioScience. 2015;3(2):46-54. doi: 10.11648/j.ajbio.20150302.14

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@article{10.11648/j.ajbio.20150302.14,
  author = {Dmytro W. Shytikov and Maryna S. Shkumat and Tetiana M. Yankova and Alex G. Peregudov and Igor V. Artyuhov and Iryna M. Pishel},
  title = {Splenic Niche Cells from Young Heterochronic Parabionts Have Decreased Capability to Amplify T-cell Proliferation in Vitro},
  journal = {American Journal of BioScience},
  volume = {3},
  number = {2},
  pages = {46-54},
  doi = {10.11648/j.ajbio.20150302.14},
  url = {https://doi.org/10.11648/j.ajbio.20150302.14},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajbio.20150302.14},
  abstract = {Immune system dysfunction during aging is well documented fact. Changes in the adaptive immune system are the most marked, especially in the T-cell compartment. In multiple preliminary studies it has been shown that parabiosis between 2 animals of different age can induce age-related changes in adaptive immune response and T-cell subpopulation composition of younger animal. In present study we evaluated the age-related changes in functions of rapidly renewing (macrophages) and slowly renewing cells (CD11c+ DCs) cells in spleens of young and old parabionts. We observed impaired capacity of splenic adherent cells from younger heterochronic parabionts to co-stimulate proliferation of autologous T-cells in vitro up to the level of old animals. Also we observed negative effect of this splenic cell population on intracellular signaling mechanisms that regulate PHA-activated proliferation of T-cells from young animals: statistically significant decrease of NFκB p65 expression and increased expression of IκBα during early activation events. This fact suggests that splenic macrophages may be involved in the induction of age-related changes of the immune system and they can be prospective target for further investigation.},
 year = {2015}
}

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TY - JOUR
T1 - Splenic Niche Cells from Young Heterochronic Parabionts Have Decreased Capability to Amplify T-cell Proliferation in Vitro
AU - Dmytro W. Shytikov
AU - Maryna S. Shkumat
AU - Tetiana M. Yankova
AU - Alex G. Peregudov
AU - Igor V. Artyuhov
AU - Iryna M. Pishel
Y1 - 2015/03/02
PY - 2015
N1 - https://doi.org/10.11648/j.ajbio.20150302.14
DO - 10.11648/j.ajbio.20150302.14
T2 - American Journal of BioScience
JF - American Journal of BioScience
JO - American Journal of BioScience
SP - 46
EP - 54
PB - Science Publishing Group
SN - 2330-0167
UR - https://doi.org/10.11648/j.ajbio.20150302.14
AB - Immune system dysfunction during aging is well documented fact. Changes in the adaptive immune system are the most marked, especially in the T-cell compartment. In multiple preliminary studies it has been shown that parabiosis between 2 animals of different age can induce age-related changes in adaptive immune response and T-cell subpopulation composition of younger animal. In present study we evaluated the age-related changes in functions of rapidly renewing (macrophages) and slowly renewing cells (CD11c+ DCs) cells in spleens of young and old parabionts. We observed impaired capacity of splenic adherent cells from younger heterochronic parabionts to co-stimulate proliferation of autologous T-cells in vitro up to the level of old animals. Also we observed negative effect of this splenic cell population on intracellular signaling mechanisms that regulate PHA-activated proliferation of T-cells from young animals: statistically significant decrease of NFκB p65 expression and increased expression of IκBα during early activation events. This fact suggests that splenic macrophages may be involved in the induction of age-related changes of the immune system and they can be prospective target for further investigation.
VL - 3
IS - 2
ER -

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Author Information

Dmytro W. Shytikov

D. F. Chebotarev State Institute of Gerontology National Academy of Medical Sciences of Ukraine, Kyiv, Ukraine
Maryna S. Shkumat

D. F. Chebotarev State Institute of Gerontology National Academy of Medical Sciences of Ukraine, Kyiv, Ukraine
Tetiana M. Yankova

D. F. Chebotarev State Institute of Gerontology National Academy of Medical Sciences of Ukraine, Kyiv, Ukraine
Alex G. Peregudov

Institute of Biology of Aging, Moscow, Russia
Igor V. Artyuhov

Institute of Biology of Aging, Moscow, Russia
Iryna M. Pishel

D. F. Chebotarev State Institute of Gerontology National Academy of Medical Sciences of Ukraine, Kyiv, Ukraine

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APA Style

Dmytro W. Shytikov, Maryna S. Shkumat, Tetiana M. Yankova, Alex G. Peregudov, Igor V. Artyuhov, et al. (2015). Splenic Niche Cells from Young Heterochronic Parabionts Have Decreased Capability to Amplify T-cell Proliferation in Vitro. American Journal of BioScience, 3(2), 46-54. https://doi.org/10.11648/j.ajbio.20150302.14

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Dmytro W. Shytikov; Maryna S. Shkumat; Tetiana M. Yankova; Alex G. Peregudov; Igor V. Artyuhov, et al. Splenic Niche Cells from Young Heterochronic Parabionts Have Decreased Capability to Amplify T-cell Proliferation in Vitro. Am. J. BioScience 2015, 3(2), 46-54. doi: 10.11648/j.ajbio.20150302.14

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AMA Style

Dmytro W. Shytikov, Maryna S. Shkumat, Tetiana M. Yankova, Alex G. Peregudov, Igor V. Artyuhov, et al. Splenic Niche Cells from Young Heterochronic Parabionts Have Decreased Capability to Amplify T-cell Proliferation in Vitro. Am J BioScience. 2015;3(2):46-54. doi: 10.11648/j.ajbio.20150302.14

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@article{10.11648/j.ajbio.20150302.14,
  author = {Dmytro W. Shytikov and Maryna S. Shkumat and Tetiana M. Yankova and Alex G. Peregudov and Igor V. Artyuhov and Iryna M. Pishel},
  title = {Splenic Niche Cells from Young Heterochronic Parabionts Have Decreased Capability to Amplify T-cell Proliferation in Vitro},
  journal = {American Journal of BioScience},
  volume = {3},
  number = {2},
  pages = {46-54},
  doi = {10.11648/j.ajbio.20150302.14},
  url = {https://doi.org/10.11648/j.ajbio.20150302.14},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajbio.20150302.14},
  abstract = {Immune system dysfunction during aging is well documented fact. Changes in the adaptive immune system are the most marked, especially in the T-cell compartment. In multiple preliminary studies it has been shown that parabiosis between 2 animals of different age can induce age-related changes in adaptive immune response and T-cell subpopulation composition of younger animal. In present study we evaluated the age-related changes in functions of rapidly renewing (macrophages) and slowly renewing cells (CD11c+ DCs) cells in spleens of young and old parabionts. We observed impaired capacity of splenic adherent cells from younger heterochronic parabionts to co-stimulate proliferation of autologous T-cells in vitro up to the level of old animals. Also we observed negative effect of this splenic cell population on intracellular signaling mechanisms that regulate PHA-activated proliferation of T-cells from young animals: statistically significant decrease of NFκB p65 expression and increased expression of IκBα during early activation events. This fact suggests that splenic macrophages may be involved in the induction of age-related changes of the immune system and they can be prospective target for further investigation.},
 year = {2015}
}

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