Study of the Extract Activities of Buas buas Leaves (Premna pubescens) as Immunostimulant on Rats (Rattus novegicus)

Martina Restuati; Syafruddin Ilyas; Salomo Hutahaean; Herbert Sipahutar

doi:doi:10.11648/j.ajbio.20140206.19

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Study of the Extract Activities of Buas buas Leaves (Premna pubescens) as Immunostimulant on Rats (Rattus novegicus)

Martina Restuati, Syafruddin Ilyas, Salomo Hutahaean, Herbert Sipahutar

Published in American Journal of BioScience (Volume 2, Issue 6)

Received: 28 November 2014 Accepted: 14 December 2014 Published: 19 December 2014

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Abstract

Buas buas (Premna pubescens) is one of the plants in Indonesia which is believed to have medicinal properties, but it is not certain. This plant is consumed by the Malay community, one of the tribes in North Sumatra - Indonesia, as vegetables, especially during the fasting month. This paper describes the effects of ethanol extract of leaves of Buas buas as an immunostimulant in rats (Rattus norvegicus). The experiments were performed on 24 tail of male rats, which were three months old, weighing 140-180 g. There were four groups of experiment ie Group A0 was given Carboxy Methyl Cellulose mice (CMC) (control group), group A1 was given 500 mg/ kg bw of Ethanol Extracts of Buas buas. Group A2, given ethanol extracts of Buas buas 500 mg / kg bw + SRBC, and the group A3 only given Sheep Red Blood Cell (SRBC). After this experiment, apigenin levels were analyzed by High Performance Liquid Chromatography (HPLC) Agilent 1220. Then the activity of immunostimulation is determinated by measuring the leukocyte count, percentage of lymphocytes, antibody titers, the levels of immunoglobulin M (IgM), immunoglobulin G (IgG), and lysozyme with ABX Micros 60. The group A2 is seen the highest group of in the number of leukocytes, antibody titer levels, imonoglobulin G (IgG), and imonoglobulin M. The amount of lysozyme owned group A2 is 0:04 ug / ml, whereas other treatments to obtain results that are not different relative significantly. Provision of Ethanol Extracts of Buas buas on mice can increase the leukocytes, lymphocytes, antibody titers, IgM, IgG and Lysozyme.

Published in	American Journal of BioScience (Volume 2, Issue 6)
DOI	10.11648/j.ajbio.20140206.19
Page(s)	244-250
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2014. Published by Science Publishing Group

Keywords

Premna Pubescens, Titer Antibodies, Lymphocytes, SRBC, IgG, IgM, Lysozyme

References

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[5]	Fang, J., Xia, C., Cao. Z., Zheng, J.Z., Reed, E., dan Jiang, B.H. 2005. Apigenin inhibits VEGF and HIF-1 expression via P13K/AKT/ p7056K1 and HDM2/p53 pathways. The FASEB journal.19. 342-353. http://dx.doi.org/10.1096/fj.04-2175com
[6]	Zheng. P.W, Chiang. L.C, Lin, C.C. 2005. Apigenin Induced Apoptosis through p53-dependent pathway in human Cervikal Carcinova Cells. Life Science. Volume 76. Issue 12. http://dx.doi.org/10.1016/j.lfs.2004.08.023
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Martina Restuati, Syafruddin Ilyas, Salomo Hutahaean, Herbert Sipahutar. (2014). Study of the Extract Activities of Buas buas Leaves (Premna pubescens) as Immunostimulant on Rats (Rattus novegicus). American Journal of BioScience, 2(6), 244-250. https://doi.org/10.11648/j.ajbio.20140206.19

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ACS Style

Martina Restuati; Syafruddin Ilyas; Salomo Hutahaean; Herbert Sipahutar. Study of the Extract Activities of Buas buas Leaves (Premna pubescens) as Immunostimulant on Rats (Rattus novegicus). Am. J. BioScience 2014, 2(6), 244-250. doi: 10.11648/j.ajbio.20140206.19

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AMA Style

Martina Restuati, Syafruddin Ilyas, Salomo Hutahaean, Herbert Sipahutar. Study of the Extract Activities of Buas buas Leaves (Premna pubescens) as Immunostimulant on Rats (Rattus novegicus). Am J BioScience. 2014;2(6):244-250. doi: 10.11648/j.ajbio.20140206.19

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@article{10.11648/j.ajbio.20140206.19,
  author = {Martina Restuati and Syafruddin Ilyas and Salomo Hutahaean and Herbert Sipahutar},
  title = {Study of the Extract Activities of Buas buas Leaves (Premna pubescens) as Immunostimulant on Rats (Rattus novegicus)},
  journal = {American Journal of BioScience},
  volume = {2},
  number = {6},
  pages = {244-250},
  doi = {10.11648/j.ajbio.20140206.19},
  url = {https://doi.org/10.11648/j.ajbio.20140206.19},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajbio.20140206.19},
  abstract = {Buas buas (Premna pubescens) is one of the plants in Indonesia which is believed to have medicinal properties, but it is not certain. This plant is consumed by the Malay community, one of the tribes in North Sumatra - Indonesia, as vegetables, especially during the fasting month. This paper describes the effects of ethanol extract of leaves of Buas buas as an immunostimulant in rats (Rattus norvegicus). The experiments were performed on 24 tail of male rats, which were three months old, weighing 140-180 g. There were four groups of experiment ie Group A0 was given Carboxy Methyl Cellulose mice (CMC) (control group), group A1 was given 500 mg/ kg bw of Ethanol Extracts of Buas buas. Group A2, given ethanol extracts of Buas buas 500 mg / kg bw + SRBC, and the group A3 only given Sheep Red Blood Cell (SRBC). After this experiment, apigenin levels were analyzed by High Performance Liquid Chromatography (HPLC) Agilent 1220. Then the activity of immunostimulation is determinated by measuring the leukocyte count, percentage of lymphocytes, antibody titers, the levels of immunoglobulin M (IgM), immunoglobulin G (IgG), and lysozyme with ABX Micros 60. The group A2 is seen the highest group of in the number of leukocytes, antibody titer levels, imonoglobulin G (IgG), and imonoglobulin M. The amount of lysozyme owned group A2 is 0:04 ug / ml, whereas other treatments to obtain results that are not different relative significantly. Provision of Ethanol Extracts of Buas buas on mice can increase the leukocytes, lymphocytes, antibody titers, IgM, IgG and Lysozyme.},
 year = {2014}
}

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TY - JOUR
T1 - Study of the Extract Activities of Buas buas Leaves (Premna pubescens) as Immunostimulant on Rats (Rattus novegicus)
AU - Martina Restuati
AU - Syafruddin Ilyas
AU - Salomo Hutahaean
AU - Herbert Sipahutar
Y1 - 2014/12/19
PY - 2014
N1 - https://doi.org/10.11648/j.ajbio.20140206.19
DO - 10.11648/j.ajbio.20140206.19
T2 - American Journal of BioScience
JF - American Journal of BioScience
JO - American Journal of BioScience
SP - 244
EP - 250
PB - Science Publishing Group
SN - 2330-0167
UR - https://doi.org/10.11648/j.ajbio.20140206.19
AB - Buas buas (Premna pubescens) is one of the plants in Indonesia which is believed to have medicinal properties, but it is not certain. This plant is consumed by the Malay community, one of the tribes in North Sumatra - Indonesia, as vegetables, especially during the fasting month. This paper describes the effects of ethanol extract of leaves of Buas buas as an immunostimulant in rats (Rattus norvegicus). The experiments were performed on 24 tail of male rats, which were three months old, weighing 140-180 g. There were four groups of experiment ie Group A0 was given Carboxy Methyl Cellulose mice (CMC) (control group), group A1 was given 500 mg/ kg bw of Ethanol Extracts of Buas buas. Group A2, given ethanol extracts of Buas buas 500 mg / kg bw + SRBC, and the group A3 only given Sheep Red Blood Cell (SRBC). After this experiment, apigenin levels were analyzed by High Performance Liquid Chromatography (HPLC) Agilent 1220. Then the activity of immunostimulation is determinated by measuring the leukocyte count, percentage of lymphocytes, antibody titers, the levels of immunoglobulin M (IgM), immunoglobulin G (IgG), and lysozyme with ABX Micros 60. The group A2 is seen the highest group of in the number of leukocytes, antibody titer levels, imonoglobulin G (IgG), and imonoglobulin M. The amount of lysozyme owned group A2 is 0:04 ug / ml, whereas other treatments to obtain results that are not different relative significantly. Provision of Ethanol Extracts of Buas buas on mice can increase the leukocytes, lymphocytes, antibody titers, IgM, IgG and Lysozyme.
VL - 2
IS - 6
ER -

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Author Information

Martina Restuati

Biology Education Department, Faculty of Mathematic and Science, State University of Medan, Medan, Indonesia
Syafruddin Ilyas

Biology Department, Faculty of Mathematic and Science, University of North Sumatra, Medan, Indonesia
Salomo Hutahaean

Biology Department, Faculty of Mathematic and Science, University of North Sumatra, Medan, Indonesia
Herbert Sipahutar

Biology Education Department, Faculty of Mathematic and Science, State University of Medan, Medan, Indonesia

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APA Style

Martina Restuati, Syafruddin Ilyas, Salomo Hutahaean, Herbert Sipahutar. (2014). Study of the Extract Activities of Buas buas Leaves (Premna pubescens) as Immunostimulant on Rats (Rattus novegicus). American Journal of BioScience, 2(6), 244-250. https://doi.org/10.11648/j.ajbio.20140206.19

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ACS Style

Martina Restuati; Syafruddin Ilyas; Salomo Hutahaean; Herbert Sipahutar. Study of the Extract Activities of Buas buas Leaves (Premna pubescens) as Immunostimulant on Rats (Rattus novegicus). Am. J. BioScience 2014, 2(6), 244-250. doi: 10.11648/j.ajbio.20140206.19

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AMA Style

Martina Restuati, Syafruddin Ilyas, Salomo Hutahaean, Herbert Sipahutar. Study of the Extract Activities of Buas buas Leaves (Premna pubescens) as Immunostimulant on Rats (Rattus novegicus). Am J BioScience. 2014;2(6):244-250. doi: 10.11648/j.ajbio.20140206.19

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@article{10.11648/j.ajbio.20140206.19,
  author = {Martina Restuati and Syafruddin Ilyas and Salomo Hutahaean and Herbert Sipahutar},
  title = {Study of the Extract Activities of Buas buas Leaves (Premna pubescens) as Immunostimulant on Rats (Rattus novegicus)},
  journal = {American Journal of BioScience},
  volume = {2},
  number = {6},
  pages = {244-250},
  doi = {10.11648/j.ajbio.20140206.19},
  url = {https://doi.org/10.11648/j.ajbio.20140206.19},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajbio.20140206.19},
  abstract = {Buas buas (Premna pubescens) is one of the plants in Indonesia which is believed to have medicinal properties, but it is not certain. This plant is consumed by the Malay community, one of the tribes in North Sumatra - Indonesia, as vegetables, especially during the fasting month. This paper describes the effects of ethanol extract of leaves of Buas buas as an immunostimulant in rats (Rattus norvegicus). The experiments were performed on 24 tail of male rats, which were three months old, weighing 140-180 g. There were four groups of experiment ie Group A0 was given Carboxy Methyl Cellulose mice (CMC) (control group), group A1 was given 500 mg/ kg bw of Ethanol Extracts of Buas buas. Group A2, given ethanol extracts of Buas buas 500 mg / kg bw + SRBC, and the group A3 only given Sheep Red Blood Cell (SRBC). After this experiment, apigenin levels were analyzed by High Performance Liquid Chromatography (HPLC) Agilent 1220. Then the activity of immunostimulation is determinated by measuring the leukocyte count, percentage of lymphocytes, antibody titers, the levels of immunoglobulin M (IgM), immunoglobulin G (IgG), and lysozyme with ABX Micros 60. The group A2 is seen the highest group of in the number of leukocytes, antibody titer levels, imonoglobulin G (IgG), and imonoglobulin M. The amount of lysozyme owned group A2 is 0:04 ug / ml, whereas other treatments to obtain results that are not different relative significantly. Provision of Ethanol Extracts of Buas buas on mice can increase the leukocytes, lymphocytes, antibody titers, IgM, IgG and Lysozyme.},
 year = {2014}
}

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